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首页> 外文期刊>Osteoarthritis and cartilage >Clinical outcome of autologous chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters
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Clinical outcome of autologous chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters

机译：自体软骨细胞植入的临床结局与红外光谱成像参数相关

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Objective: To investigate whether Fourier transform infrared imaging spectroscopy (FT-IRIS), a modality based on molecular vibrations, is a viable alternative to histology and immunohistochemistry (IHC) for assessment of tissue quality and patient clinical outcome. Methods: Osteochondral biopsies were obtained from patients (9-65. months post-surgery) who underwent an autologous chondrocyte implantation (ACI) procedure to repair a cartilage defect (N = 14). The repair tissue was evaluated histologically by OsScore grading, for the presence of types I and II collagen by IHC, and for proteoglycan (PG) distribution and collagen quality parameters by FT-IRIS. Patient clinical outcome was assessed by the Lysholm score. Results: Improvement in Lysholm score occurred in 79% of patients. IHC staining showed the presence of types I and II collagen in all samples, with a greater amount of collagen type II in the deep zone. The amount and location of immunostaining for type II collagen correlated to the FT-IRIS-derived parameters of relative PG content and collagen helical integrity. In addition, the improvement in Lysholm score post-ACI correlated positively with the OsScore, type II collagen (IHC score) and FT-IRIS-determined parameters. Regression models for the relation between improvement in Lysholm score and either OsScore, IHC area score or the FT-IRIS parameters all reached significance (p < 0.01). However, the FT-IRIS model was not significantly improved with inclusion of the OsScore and IHC score parameters. Conclusion: Demonstration of the correlation between FT-IRIS-derived molecular parameters of cartilage repair tissue and patient clinical outcome lays the groundwork for translation of this methodology to the clinical environment to aid in the management of cartilage disorders and their treatment.
机译：目的：研究基于分子振动的傅里叶变换红外成像光谱法（FT-IRIS）是否可以替代组织学和免疫组织化学（IHC）来评估组织质量和患者临床疗效。方法：骨软骨活检取自术后（9-65。个月）接受自体软骨细胞植入（ACI）手术修复软骨缺损（N = 14）的患者。通过OsScore分级在组织学上评估修复组织，通过IHC评估I和II型胶原蛋白的存在，并通过FT-IRIS评估蛋白聚糖（PG）的分布和胶原蛋白的质量参数。通过Lysholm评分评估患者的临床结局。结果：79％的患者的Lysholm评分得到改善。 IHC染色显示所有样品中均存在I型和II型胶原，深部区域中II型胶原的含量更高。 II型胶原蛋白的免疫染色的数量和位置与FT-IRIS衍生的相对PG含量和胶原蛋白螺旋完整性的参数有关。此外，ACI后Lysholm评分的改善与OsScore，II型胶原蛋白（IHC评分）和FT-IRIS确定的参数呈正相关。 Lysholm评分改善与OsScore，IHC面积评分或FT-IRIS参数之间关系的回归模型均达到显着性（p <0.01）。但是，FT-IRIS模型没有包含OsScore和IHC评分参数得到显着改善。结论：FT-IRIS衍生的软骨修复组织分子参数与患者临床结果之间相关性的证明，为将该方法转换为临床环境奠定了基础，以帮助管理软骨疾病及其治疗。

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