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首页> 外文期刊>Osteoarthritis and cartilage >Human aggrecanase generated synovial fluid fragment levels are elevated directly after knee injuries due to proteolysis both in the inter globular and chondroitin sulfate domains.
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Human aggrecanase generated synovial fluid fragment levels are elevated directly after knee injuries due to proteolysis both in the inter globular and chondroitin sulfate domains.

机译：膝关节受伤后，由于球蛋白间和硫酸软骨素域中的蛋白水解作用，人类软骨聚集蛋白聚糖酶生成的滑液碎片水平直接升高。

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OBJECTIVE: To examine different aggrecanase generated fragments in synovial fluid (SF) from patients with acute and chronic knee injuries and from knee healthy subjects. METHODS: We prepared SF-D1 samples from acute (n=35) and chronic (n=35) knee injury patients and knee healthy subjects (n=10). Aggrecan fragments were analyzed in the SF-D1 samples by quantitative (G1, ARGS, KEEE and G3 antibodies) and non-quantitative (GRGT and AGEG antibodies) Western blot. RESULTS: ARGS-SELE, ARGS-chondroitin sulfate (CS)1, GRGT-, GLGS- and AGEG-G3 fragments were the main ARGS and G3 fragments in injured and reference samples. In the acute injury samples the concentrations of these fragments were increased compared to the reference, and the level of the ARGS-SELE remained elevated for at least 2 years after the joint injury. Both SF ARGS fragments and aggrecanase generated G3 fragments had high sensitivity and specificity as biomarkers in distinguishing injured from healthy knee joints, although the ARGS fragments had higher area under the receiver operating characteristic curve (AUC) values for injuries (74-86%) than the G3 fragments (AUC values 63-68%). CONCLUSION: Our results suggest that during the acute phase after knee injury there is an increased aggrecanase activity against both the interglobular domain (IGD) and the CS2 cleavage sites of joint cartilage aggrecan. This increase in SF aggrecanolytic fragments is present for several years after the injury. SF ARGS fragments are better biomarkers than the aggrecanase generated G3-fragments in distinguishing injured from healthy knee joints.
机译：目的：研究急性和慢性膝关节损伤患者以及膝关节健康受试者滑膜液中不同的软骨聚集蛋白聚糖酶生成的片段。方法：我们从急性（n = 35）和慢性（n = 35）膝关节损伤患者以及膝关节健康受试者（n = 10）中制备了SF-D1样品。通过定量（G1，ARGS，KEEE和G3抗体）和非定量（GRGT和AGEG抗体）Western印迹分析SF-D1样品中的Aggrecan片段。结果：ARGS-SELE，ARGS-硫酸软骨素（CS）1，GRGT-，GLGS-和AGEG-G3片段是受伤和参考样品中的主要ARGS和G3片段。与参考相比，在急性损伤样品中这些碎片的浓度有所增加，并且在关节损伤后至少2年内，ARGS-SELE的水平仍保持升高。 SF ARGS片段和软骨聚集蛋白聚糖酶生成的G3片段均具有较高的敏感性和特异性，可作为区分健康膝关节损伤的生物标志物，尽管ARGS片段在损伤的接受者操作特征曲线（AUC）值以下具有较高的面积（74-86％） G3片段（AUC值为63-68％）。结论：我们的结果表明，在膝关节损伤后的急性期中，针对关节软骨聚集蛋白聚糖的球间域（IGD）和CS2裂解位点的聚集蛋白聚糖酶活性均增加。受伤后数年以来，SF聚集蛋白水解片段的这种增加一直存在。 SF ARGS片段在区分健康膝关节损伤方面比聚集蛋白聚糖酶产生的G3片段更好。

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