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首页> 外文期刊>Osteoarthritis and cartilage >Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation.
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Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation.

机译：一氧化氮参与软骨骨赘形成的软骨细胞死亡。

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OBJECTIVE: To examine the nitric oxide (NO) production relevant to chondrocyte cell death in order to elucidate the mechanism of chondro-osteophyte formation in osteoarthrotic joints. DESIGN: Human chondro-osteophytes were obtained during total hip arthroplasty. Expression of inducible nitric oxide synthase (iNOS) mRNA was determined by in-situ hybridization. Localization of iNOS and nitrotyrosine at protein level were examined by immunohistochemistry. Cell death of chondrocytes were confirmed by both TUNEL method and transmission electron microscopy. RESULTS: The various populations of proliferative and hypertrophic chondrocytes expressed iNOS mRNA and iNOS as well as nitrotyrosine protein. Approximately 30% of hypertrophic chondrocytes forming chondro-osteophyte showed positive reaction to TUNEL staining. Electron microscopy confirmed both disintegrated and apoptotic chondrocytes in these zones. In the deep hypertrophic zone calcification was seen around each of the matrix vesicles and some masses of cell debris. CONCLUSION: Chondro-osteophyte formation involves NO production by chondrocytes. The expression and localization of iNOS and nitrotyrosine in chondro-osteophytes suggest the significant role of NO in chondrocyte hypertrophy and apoptosis. Copyright 2001 OsteoArthritis Research Society International.
机译：目的：研究与软骨细胞死亡相关的一氧化氮（NO）产生，以阐明骨关节炎关节中软骨骨赘形成的机制。设计：在全髋关节置换术中获得了人类软骨骨赘。通过原位杂交确定诱导型一氧化氮合酶（iNOS）mRNA的表达。通过免疫组织化学检查了iNOS和硝基酪氨酸在蛋白质水平上的定位。 TUNEL法和透射电镜均证实了软骨细胞的死亡。结果：各种增殖和肥大软骨细胞群体均表达了iNOS mRNA和iNOS以及硝基酪氨酸蛋白。约有30％的形成软骨骨赘的肥大软骨细胞对TUNEL染色呈阳性反应。电子显微镜检查证实了这些区域中的软骨细胞解体和凋亡。在肥厚的深部区域，每个基质囊泡和一些细胞碎片团块都可见钙化。结论：软骨骨赘的形成涉及软骨细胞不产生NO。 iNOS和硝基酪氨酸在软骨骨赘中的表达和定位提示NO在软骨细胞肥大和凋亡中的重要作用。版权所有2001国际骨关节炎研究协会。

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