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首页> 外文期刊>Osteoarthritis and cartilage >Increased cellular infiltrate in inflammatory synovia of osteoarthritic knees.
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Increased cellular infiltrate in inflammatory synovia of osteoarthritic knees.

机译：骨关节炎性膝关节炎性滑膜细胞浸润增加。

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OBJECTIVE: To determine the nature and origin of synovial inflammation in knees with osteoarthritis (OA). METHOD: Synovial samples were obtained from 21 medial compartmental knee OAs from 19 patients. First, using 11 medial knee synovial samples from 9 patients, a quantitative estimation of synovitis was made with both ordinary and immunohistochemical staining. Second, from the other 10 knees, tissue samples were taken from both the medial and the lateral compartments to quantify cells that infiltrated into the synovium. Medial synovium was immunostained using antibodies to anti-type II collagen, CD68, CD2, CD4, CD8, CD15, CD19, CD25, HLA-DR, CD1a and LN5. The lateral synovium was immunostained with anti-type II collagen, CD68, HLA-DR and CD4 antibody as a control. RESULT: Denatured cartilaginous detritus was found captured by synovial lining cells with a strong immunoreactivity to CD68 antibody, and whose phagocytic potential was activated. The number of anti-type II collagen-positive fragments in the medial compartment of the knee was larger than that found in the lateral compartment. Moreover, the population of CD68-positive cells in synovial tissue and HLA-DR-positive cells in the lining layer was larger in the medial compartment than in the lateral compartment. The number of CD4-positive cells (defined as helper/inducer T lymphocytes) was greater in medial synovium than in lateral synovium. CONCLUSION: Overall, this study strongly supports the concept that the synovitis observed in patients with knee OA might be induced by an immunological mechanism involving, to some extent, a macrophage/helper T cell interaction. Copyright 2002 OsteoArthritis Research Society International.
机译：目的：确定骨关节炎（OA）膝关节滑膜炎症的性质和起源。方法：滑膜样本取自19例患者的21个内侧房室OA。首先，使用来自9位患者的11个内侧膝关节滑膜样本，通过常规和免疫组织化学染色对滑膜炎进行定量评估。其次，从其他10个膝盖的内侧和外侧隔室中抽取组织样本，以量化渗入滑膜的细胞。使用抗II型胶原蛋白，CD68，CD2，CD4，CD8，CD15，CD19，CD25，HLA-DR，CD1a和LN5的抗体对内侧滑膜进行免疫染色。用抗II型胶原蛋白，CD68，HLA-DR和CD4抗体作为对照对外侧滑膜进行免疫染色。结果：发现变性的软骨碎屑被对CD68抗体具有强免疫反应性的滑膜衬里细胞捕获，并且其吞噬能力被激活。膝关节内侧腔室中抗II型胶原蛋白阳性片段的数量大于外侧腔室中的数量。此外，滑膜组织中CD68阳性细胞和衬层中HLA-DR阳性细胞的数量在内侧室比外侧室要大。内侧滑膜中的CD4阳性细胞（定义为辅助/诱导性T淋巴细胞）的数量大于外侧滑膜。结论：总的来说，这项研究强烈支持这样的观念，即在膝OA患者中观察到的滑膜炎可能是由某种程度上涉及巨噬细胞/辅助T细胞相互作用的免疫机制诱导的。版权所有2002 OsteoArthritis Research Society International。

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