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首页> 外文期刊>Online journal of veterinary research OJVR >Isolation and identification of a new isolate of avian infectious bronchitisvirus IRFIBV32 and a study of its pathogenicity
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Isolation and identification of a new isolate of avian infectious bronchitisvirus IRFIBV32 and a study of its pathogenicity

机译：禽传染性支气管炎病毒IRFIBV32新分离株的分离鉴定及其致病性研究

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摘要

Infectious bronchitis (IB)is one of the most significant highly acute viral respiratory diseases of commercialchickens in the world. We isolated, identified and studied the pathogenicity of anisolate of IB virus (IRFIBV32) from broiler chicken farms in Fars province. Trachealtissue samples of a field outbreak of respiratory disease in commercial broilers wereprocessed for virus identification. The 5' part of the S1 gene of the spike protein wasamplified and sequenced. The nucleotide sequence was found to have identity (95 %)with 793/B strains of avian infectious bronchitis virus. The isolate produced typicalembryo lesions including curling and stunting, suggestive of infectious bronchitisvirus infection after three passages. Infected allantoic fluids were tested by nestedRT-PCR and then, ELD50 was calculated. Tracheal ciliary activity using carboniferouspigment granules in infected chicken inoculated by 105ELD50/ml of the virus wasevaluated. The results showed the isolate could induce ciliastasis. Experimentallyaffected chicks showed mild respiratory rales and difficult breathing. The mostprominent gross lesions were observed in the trachea and kidneys of experimentallyinfected chickens.
机译：传染性支气管炎（IB）是世界上最重要的商业鸡高度急性病毒性呼吸道疾病之一。我们从法尔斯省的肉鸡场中分离，鉴定和研究了IB病毒分离株（IRFIBV32）的致病性。对商业肉鸡呼吸道疾病现场暴发的气管组织样本进行了处理，以进行病毒鉴定。刺突蛋白的S1基因的5'部分被扩增并测序。发现该核苷酸序列与禽传染性支气管炎病毒的793 / B株具有同一性（95％）。分离物产生典型的胚胎损伤，包括卷曲和发育迟缓，提示经过三代后感染性支气管炎病毒感染。通过巢式RT-PCR检测感染的尿囊液，然后计算ELD50。使用石炭纪色素颗粒对感染了105ELD50 / ml病毒的鸡进行气管睫毛活性评估。结果表明该分离株可引起纤毛停滞。受实验影响的小鸡表现出轻度的呼吸音和呼吸困难。在实验感染鸡的气管和肾脏中观察到最明显的肉眼病变。

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