首页> 外文期刊>Otolaryngology--head and neck surgery: official journal of American Academy of Otolaryngology-Head and Neck Surgery >Role of anaerobes in chronic sinusitis: Will polymerase chain reaction solve the debate.
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Role of anaerobes in chronic sinusitis: Will polymerase chain reaction solve the debate.

机译:厌氧菌在慢性鼻窦炎中的作用:聚合酶链反应能否解决这一争论。

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BACKGROUND: Bacteriology of chronic sinusitis continues to be a matter of debate, particularly the role of anaerobes. Some authors suggest that anaerobes play a significant role whereas others suggest a minimal role. Those who suggest a significant role argue that standard culture techniques are the culprits. OBJECTIVE: The purpose of this study was to use polymerase chain reaction (PCR) on sinus specimens for the presence of anaerobes and to compare them with standard culture techniques. METHODS: Sixty-four samples were obtained in a sterile fashion during sinus surgery and were sent for standard anaerobic cultures as well as anaerobic PCR analysis. RESULTS: Anaerobic bacteria were demonstrated in 5% of culture specimens, which is similar to recently published data. PCR identified anaerobic bacteria in 19% of the specimens (P = 0.01). CONCLUSION: PCR analysis of surgical samples obtained during endoscopic sinus surgery for chronic sinusitis identified a significantly higher incidence of anaerobes (x4) compared with standard anaerobic culture technique.
机译:背景:慢性鼻窦炎的细菌学仍然是一个有争议的问题,尤其是厌氧菌的作用。一些作者建议厌氧菌起重要作用,而另一些人建议起最小作用。那些建议扮演重要角色的人认为标准的养殖技术是罪魁祸首。目的:本研究的目的是对鼻窦标本使用聚合酶链反应(PCR)检测厌氧菌的存在,并将其与标准培养技术进行比较。方法:在鼻窦手术中以无菌方式获得了64个样本,并将其送至标准厌氧培养以及厌氧PCR分析。结果:5%的培养标本中显示出厌氧细菌,这与最近发表的数据相似。 PCR在19%的标本中鉴定出厌氧细菌(P = 0.01)。结论:内窥镜鼻窦手术治疗慢性鼻窦炎的手术样品的PCR分析表明,与标准厌氧培养技术相比,厌氧菌(x4)的发生率显着更高。

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