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首页> 外文期刊>Cell and tissue banking: An international journal of banking, engineering & transplantation of cells and tissues >Effect of hydrogen peroxide on human tendon allograft
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Effect of hydrogen peroxide on human tendon allograft

机译：过氧化氢对同种异体肌腱移植的影响

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摘要

Bacterial contamination of tendon allografts at the completion of processing has historically been about 2 %, with tendons that are found to be culture positive being discarded. Treatment of tendon allograft with hydrogen peroxide at the beginning of tissue processing may reduce bacterial contamination, however, the potential side effects of hydrogen peroxide treatment include hydrolysis of the collagen and this may alter the mechanical properties of the graft. Pairs of human tendons were used. One was washed in 3 % hydrogen peroxide for 5 min and the untreated tendon was used as a control. The ultimate tensile strength of the tendons was determined using a material testing machine. A freeze clamp technique was used to hold the tendons securely at the high loads required to cause tendon failure. There was no statistical difference in the ultimate tensile strength between the treated and untreated tendons. Mean strength ranged from Extensor Hallucis Longus at 588 Newtons to Tibialis Posterior at 2,366 Newtons. Hydrogen peroxide washing may reduce bacterial contamination of tendon allograft and does not affect the strength of the tendon.
机译：历史上，完成同种异体肌腱的细菌污染历史上约为2％，发现是培养阳性的肌腱将被丢弃。在组织处理开始时用过氧化氢处理肌腱同种异体移植物可减少细菌污染，但是过氧化氢处理的潜在副作用包括胶原蛋白的水解，这可能会改变移植物的机械性能。使用了成对的人类肌腱。将其中一个在3％过氧化氢中洗涤5分钟，然后将未处理的肌腱用作对照。肌腱的极限抗拉强度是使用材料测试机确定的。使用冷冻钳技术将肌腱牢固地保持在导致肌腱衰竭所需的高载荷下。在处理过的和未处理过的肌腱之间，极限抗拉强度没有统计学差异。平均强度范围从588牛顿的伸肌拇长肌到2,366牛顿的胫后肌。过氧化氢洗涤可以减少同种异体肌腱的细菌污染，并且不影响肌腱的强度。

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