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首页> 外文期刊>Otolaryngology--head and neck surgery: official journal of American Academy of Otolaryngology-Head and Neck Surgery >Preliminary study on the stability of beta-2 transferrin in extracorporeal cerebrospinal fluid.
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Preliminary study on the stability of beta-2 transferrin in extracorporeal cerebrospinal fluid.

机译：β-2转铁蛋白在体外脑脊液中稳定性的初步研究。

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OBJECTIVE: Detection of beta-2 transferrin in rhinorrhea fluid is a sensitive and specific method for the diagnosis of a cerebrospinal fluid (CSF) leak. Patients may be asked to collect this fluid at home to obtain an adequate volume for detection, and thus the age and storage conditions of these specimens may be variable upon analysis. The purpose of this study is to understand how age, storage temperature, and exposure to mucus affect the ability to detect beta-2 transferrin in CSF. STUDY DESIGN: Case series with planned data collection. SETTING: Tertiary care university hospital. SUBJECTS AND METHODS: This study consists of 6 patients undergoing endoscopic CSF leak repair. CSF was collected directly from a lumbar drain (n = 4) or from nasal drainage (n = 2). Specimens were stored at 4 degrees C (n = 3) or room temperature (n = 3). Samples were tested for the presence of beta-2 transferrin for up to 7 days using standard immunofixation electrophoresis techniques. RESULTS: Beta-2 transferrin was detected in all specimens through day 7 regardless of storage temperature or collection site (95% exact binomial confidence interval of 0%-46%). CONCLUSIONS: Beta-2 transferrin remains detectable in extracorporeal CSF for up to 7 days regardless of storage at room temperature or exposure to nasal mucus. Negative detection in patient specimens up to a week old is therefore not likely to be caused by protein degradation.
机译：目的：检测鼻漏液中的β-2转铁蛋白是诊断脑脊液（CSF）泄漏的灵敏且特异的方法。可能会要求患者在家中收集这种液体以获得足够的检测体积，因此这些样本的年龄和储存条件在分析时可能会有所不同。这项研究的目的是了解年龄，储存温度和粘液暴露如何影响检测CSF中β-2转铁蛋白的能力。研究设计：具有计划数据收集的病例系列。地点：三级护理大学医院。研究对象和方法：本研究由6例接受内镜CSF漏诊修复的患者组成。脑脊液直接从腰部引流（n = 4）或鼻腔引流（n = 2）收集。标本保存在4摄氏度（n = 3）或室温下（n = 3）。使用标准免疫固定电泳技术测试样品中是否存在β-2转铁蛋白长达7天。结果：到第7天为止，所有样品中均检测到Beta-2转铁蛋白，无论其储存温度或收集地点如何（95％准确的二项式置信区间为0％-46％）。结论：无论在室温下保存或暴露于鼻粘液中，β-2转铁蛋白在体外脑脊液中均可检测长达7天。因此，长达一周的患者样本中的阴性检测不太可能是由于蛋白质降解引起的。

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