首页> 外文期刊>Russian Journal of Developmental Biology >Different Influence of Mutant Alleles of the APETALA1 Gene on the Development of Reproductive Organs in abruptus Mutant Flowers of the of Arabidopsis thaliana (L.) Heynh.
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Different Influence of Mutant Alleles of the APETALA1 Gene on the Development of Reproductive Organs in abruptus Mutant Flowers of the of Arabidopsis thaliana (L.) Heynh.

机译:APETALA1基因突变等位基因对拟南芥突变体突变花中生殖器官发育的不同影响。

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摘要

The APETALA1 (AP1) gene of A. thaliana codes type II MADS protein with domains MADS, I, K, and C. The role of K-and C-domains in the functioning of AP1 protein is poorly investigated. The analysis of phenotypic manifestation of mutations disrupting the activity of various domains of the protein product allows one to obtain information on the function of domains and, thereby, on the structural-functional organization of the gene. We investigated the action of mutant alleles of the AP1 gene whose protein products are probably lacking the functionally active domains K (ap1-20), K-and C-domains (ap1-1 and ap1-6), and C-domain (ap1-3) on the flower morphology in abr mutant (the ABRUPTUS/ PINOID gene allele). It was detected that, unlike the ap1-20 allele, the presence of ap1-3, ap1-6, and ap1-1 alleles results in reduction of a number of the generative organs in the flowers of the double mutants abr ap1-3, abr ap1-6, and abr ap1-1. It was suggested that C-domain of the AP1 protein prevents the alteration of determination of the type of reproductive organs when the AP1 gene ectropically expressed in the inner whorls of a flower in the abr mutant.
机译:拟南芥的APETALA1(AP1)基因编码具有域MADS,I,K和C的II型MADS蛋白。对K和C结构域在AP1蛋白功能中的作用的研究很少。对破坏蛋白质产物各个结构域活性的突变的表型表现的分析使人们可以获得有关结构域功能的信息,从而获得有关基因的结构功能组织的信息。我们调查了AP1基因突变等位基因的作用,其蛋白产物可能缺少功能性活性域K(ap1-20),K和C域(ap1-1和ap1-6)和C域(ap1) -3)关于abr突变体(ABRUPTUS / PINOID基因等位基因)的花朵形态。已检测到,与ap1-20等位基因不同,ap1-3,ap1-6和ap1-1等位基因的存在导致双突变abr ap1-3的花朵中许多生殖器官的减少, abr ap1-6和abr ap1-1。有人提出,当abr突变体中的AP1基因在花的内部轮轴上异常表达时,AP1蛋白的C结构域阻止了生殖器官类型测定的改变。

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