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首页> 外文期刊>Russian Journal of Developmental Biology >High molecular weight protein detected in higher plant cells by antibodies against dynein is associated with vesicular organelles including Golgi apparatus
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High molecular weight protein detected in higher plant cells by antibodies against dynein is associated with vesicular organelles including Golgi apparatus

机译：抗动力蛋白抗体在高等植物细胞中检测到的高分子量蛋白与包括高尔基体在内的囊泡细胞器有关

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摘要

The cytoplasmic dynein is a multisubunit complex driving organelles along microtubules to their minus-end. We used antibodies against two functional domains (motor and microtubule-binding) of one of principal components of the complex-dynein heavy chain of slime mould Dictyostelium discoideum-to test root meristem cells of wheat Triticum aestivum. The antibodies reacted with a high molecular weight protein (> 500 kDa) in the total cell extract and the band recognized by the antibodies in plant extracts had a lower electrophoretic mobility than the high molecular weight band of mammalian dynein. Antibodies coupled to protein A-Sepharose precipitated the high molecular weight protein from the purified cell extracts. Immunocytochemical analysis demonstrated that the antigen recognized by antibodies against dynein heavy chains is associated with the vesicles whose localization depends on the cell cycle stage. The antigen-positive vesicles were localized to the perinuclear region in interphase and early prophase, to the spindle periphery and to spindle pole region during mitosis, and to the interzonal region in the period of fragmoplast and cell plate formation. Some antigen-positive vesicles also reacted with antibodies against Golgi protein markers. The obtained data indicate that higher plant cells contain a high molecular weight protein interacting with antibodies against the motor and microtubules-binding domains of Dictyostelium dynein heavy chain. The revealed antigen was associated with the vesicular structures in the cytoplasm including the Golgi apparatus.
机译：胞质动力蛋白是一个多亚基复合体，沿着微管向负端驱动细胞器。我们使用了针对粘液菌盘基网柄菌的复合动力蛋白重链主要成分之一的两个功能域（运动和微管结合）的抗体，以测试小麦普通小麦的根分生组织细胞。抗体与总细胞提取物中的高分子量蛋白质（> 500 kDa）反应，植物提取物中抗体识别的条带的电泳迁移率低于哺乳动物动力蛋白的高分子量条带。与蛋白质A-Sepharose偶联的抗体从纯化的细胞提取物中沉淀出高分子量蛋白质。免疫细胞化学分析表明，针对动力蛋白重链的抗体识别的抗原与囊泡有关，囊泡的定位取决于细胞周期阶段。抗原阳性囊泡在中期和前期定位于核周区域，在有丝分裂期间定位于纺锤体周边和纺锤极区域，并在脆弱塑料和细胞板形成期间定位于区域间区域。一些抗原阳性囊泡还与针对高尔基体蛋白标记的抗体反应。所获得的数据表明，高等植物细胞中含有一种高分子量蛋白质，该蛋白质与针对Dictyostelium dynein重链的运动和微管结合域的抗体相互作用。所揭示的抗原与包括高尔基体在内的细胞质中的囊泡结构相关。

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