...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Russian journal of bioorganic chemistry >Influence of distamycin A on the methylation, extraction, and formation of UV-inducible DNA-protein cross links of histone H1 in the interphase nuclei of rat liver cells
【24h】

Influence of distamycin A on the methylation, extraction, and formation of UV-inducible DNA-protein cross links of histone H1 in the interphase nuclei of rat liver cells

机译：二叠霉素A对大鼠肝细胞相间核中组蛋白H1的甲基化，提取及其形成的UV诱导DNA-蛋白质交联的影响

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The incubation in vitro of rat liver nuclei in the presence of S-adenosyl[methyl-~3H]methionine ([~3H] SAM) leads to the incorporation of a radioactive label not only into core histones H3 and H4, but also into linker histone H1. The addition of distamycin A to the incubation medium stimulates label incorporation into histone H1 by approximately six times and into histone H3 by around two times. The presence of distamycin facilitates histone H1 extraction by polyglutamic acid (poly(Glu)) and decreases UV-induced DNA-histone cross-link formation. These effects give evidence that the weakening H1-chromatin interaction by distamycin may be the result of a histone H1 position change relative toward the nucleosome and (or) a disturbance of the histone H1-H3 interactions, as these histones are exposed to additional methylation.
机译：在S-腺苷[甲基~~ 3H]蛋氨酸（[〜3H] SAM）存在下体外培养大鼠肝核导致放射性标记不仅掺入核心组蛋白H3和H4中，而且掺入接头中组蛋白H1。在培养培养基中添加双斯坦霉素A可使标记掺入组蛋白H1约6倍，并掺入组蛋白H3约2倍。 Distamycin的存在有助于通过聚谷氨酸（poly（Glu））提取组蛋白H1，并减少UV诱导的DNA-组蛋白交联形成。这些作用提供了证据，表明他莫霉素减弱的H1-染色质相互作用可能是相对于核小体的组蛋白H1位置变化和（或）组蛋白H1-H3相互作用受到干扰的结果，因为这些组蛋白暴露于其他甲基化状态。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号