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首页> 外文期刊>Scandinavian journal of plastic and reconstructive surgery and hand surgery >Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation.
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Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation.

机译：将碱性成纤维细胞生长因子掺入融合前的培养皮肤替代物中，以加速移植后的新生血管形成和皮肤重建。

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摘要

Cultured skin substitutes (CSS) with both epidermal and dermal components seem to be ideal, but they have not been widely used clinically, partly because it takes several weeks to produce them. Decreasing the number of seeding cells may reduce the period required for production, but it still takes a long time before the cells become confluent and neovascularisation is completed in CSS after grafting. As we have already succeeded in reducing the number of seeded keratinocytes in this study, we first attempted to reduce the number of seeded fibroblasts. Consequently, preconfluent CSS with 10 x 10(3) cells/cm2 of fibroblasts combined with 100 x 10(3) cells/cm2 of keratinocytes could be successfully grafted on to full-thickness wounds. bFGF-impregnated gelatin microspheres were then added to the preconfluent CSS before grafting. Incorporation of bFGF significantly accelerated neovascularisation and increased epidermal thickness, cellular components, and thickness of the dermis. The incorporation of bFGF makes CSS a potential therapeutic approach for management of skin wounds.
机译：具有表皮和真皮成分的培养的皮肤替代品（CSS）似乎是理想的，但是它们尚未在临床上得到广泛使用，部分原因是生产它们需要花费数周的时间。减少播种细胞的数量可能会缩短生产所需的时间，但是在移植后在CSS中使细胞融合并完成新血管形成仍需要很长时间。由于在这项研究中我们已经成功减少了种子角化细胞的数量，因此我们首先尝试减少种子成纤维细胞的数量。因此，具有10 x 10（3）细胞/ cm2成纤维细胞结合100 x 10（3）细胞/ cm2角质形成细胞的融合前CSS可以成功移植到全层伤口上。然后在移植前将bFGF浸渍的明胶微球添加到融合前的CSS中。 bFGF的掺入可显着加速新血管形成，并增加表皮厚度，细胞成分和真皮厚度。 bFGF的掺入使CSS成为治疗皮肤伤口的潜在治疗方法。

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