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首页> 外文期刊>Russian journal of electrochemistry >Efficiency of bioelectrocatalytic oxidation of ethanol by whole cells and membrane fractions of Gluconobacter Oxydans bacteria in the presence of mediators of ferrocene series
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Efficiency of bioelectrocatalytic oxidation of ethanol by whole cells and membrane fractions of Gluconobacter Oxydans bacteria in the presence of mediators of ferrocene series

机译:二茂铁系列介质存在下,氧化葡糖杆菌的全细胞和膜部分对乙醇的生物电催化氧化效率

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摘要

Bioelectrocatalytic oxidation of ethanol by whole cells and membrane fraction of Gluconobacter oxydans bacteria is studied on modified graphite-paste electrodes in mediator biosensors. Ferrocene derivatives are used as electron transport mediators for effective coupling of enzymatic and electrochemical processes on graphite electrodes. Electrochemical kinetics of the processes are studied; the obtained data are interpreted in the terms of the mechanism of two-substrate enzymatic reaction. It is shown that mediators of ferrocene series are promising compounds for development of mediator biosensors based both on whole cells of Gluconobacter oxydans bacteria and on membrane fractions of these bacteria. Bioelectrocatalytic processes of ethanol oxidation on graphite paste electrodes occur more efficiently when the bacterial membrane fraction is used as a biocatalyst and ferrocenemonocarboxylic acid is used as a mediator.
机译:在介体生物传感器的修饰石墨糊电极上研究了氧化葡糖杆菌的全细胞和膜部分对乙醇的生物电催化氧化作用。二茂铁衍生物用作电子传输介体,以有效地耦合酶和电化学过程在石墨电极上。研究了过程的电化学动力学。所获得的数据以两底物酶促反应的机理来解释。结果表明,二茂铁系列的介体是用于开发基于氧化葡糖杆菌细菌的全细胞和这些细菌的膜级分的介体生物传感器的有前途的化合物。当细菌膜级分用作生物催化剂和二茂铁一元羧酸用作介体时,乙醇在石墨浆料电极上的生物电催化过程会更有效地发生。

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