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首页> 外文期刊>Cell and tissue banking: An international journal of banking, engineering & transplantation of cells and tissues >Bacteriology testing of cardiovascular tissues: comparison of transport solution versus tissue testing
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Bacteriology testing of cardiovascular tissues: comparison of transport solution versus tissue testing

机译：心血管组织的细菌学测试：运输溶液与组织测试的比较

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摘要

Bacteriology testing is mandatory for quality control of recovered cardiovascular allografts (CVA). In this paper, two different bacteriology examinations (A tests) performed before tissue antibiotic decontamination were compared: transport solution filtration analysis (A1) and tissue fragment direct incubation (A2). For this purpose, 521 CVA (326 heart and 195 artery tissues) from 280 donors were collected and analyzed by the European Homograft Bank (EHB). Transport solution (A1) tested positive in 43.25 % of hearts and in 48.21 % of arteries, whereas the tissue samples (A2) tested positive in 38.34 % of hearts and 33.85 % of arteries. The main species identified in both A1 and A2 were Staphylococcus spp. in 55 and 26 % of cases, and Propionibacterium spp. in 8 and 19 %, respectively. Mismatches in bacteriology results between both initial tests A1 and A2 were found. 18.40 % of the heart valves were identified as positive by A1 whilst 13.50 % were considered positive by A2. For arteries, 20.51 % of cases were positive in A1 and negative in A2, and just 6.15 % of artery allografts presented contamination in the A2 test but were considered negative for the A1 test. Comparison between each A test with the B and C tests after antibiotic treatment of the allograft was also performed. A total decontamination rate of 70.8 % of initial positive A tests was obtained. Due to the described mismatches and different bacteria identification percentage, utilization of both A tests should be implemented in tissue banks in order to avoid false negatives.
机译：细菌学测试对于回收的心血管同种异体移植（CVA）的质量控制是必不可少的。在本文中，比较了在组织抗生素去污之前进行的两种不同的细菌学检查（A测试）：转运液过滤分析（A1）和组织碎片直接孵育（A2）。为此，欧洲同种异体移植银行（EHB）收集了280个捐献者的521 CVA（326个心脏和195个动脉组织）并进行了分析。运输溶液（A1）在43.25％的心脏和48.21％的动脉中呈阳性，而组织样本（A2）在38.34％的心脏和33.85％的动脉中呈阳性。在A1和A2中鉴定出的主要物种是葡萄球菌。在55％和26％的病例中，丙酸杆菌属。分别为8％和19％。发现初始测试A1和A2之间的细菌学结果不匹配。 A1将18.40％的心脏瓣膜鉴定为阳性，而A2则将13.50％的心脏瓣膜视为阳性。对于动脉，A1阳性的病例为20.51％，A2阴性的病例，在A2测试中仅6.15％的动脉同种异体呈污染，但在A1测试中被视为阴性。还对同种异体移植物进行了抗生素处理后，将每个A测试与B和C测试进行了比较。最初的阳性A测试总污染率为70.8％。由于所描述的不匹配和不同的细菌鉴定百分比，应在组织库中同时使用两种A测试，以避免假阴性。

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