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首页> 外文期刊>Cell and tissue banking: An international journal of banking, engineering & transplantation of cells and tissues >Characterization of pediatric microtia cartilage: a reservoir of chondrocytes for auricular reconstruction using tissue engineering strategies
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Characterization of pediatric microtia cartilage: a reservoir of chondrocytes for auricular reconstruction using tissue engineering strategies

机译：小儿小口软骨的特征：使用组织工程策略进行耳廓重建的软骨细胞贮存库

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The external ear is composed of elastic cartilage. Microtia is a congenital malformation of the external ear that involves a small reduction in size or a complete absence. The aim of tissue engineering is to regenerate tissues and organs clinically implantable based on the utilization of cells and biomaterials. Remnants from microtia represent a source of cells for auricular reconstruction using tissue engineering. To examine the macromolecular architecture of microtia cartilage and behavior of chondrocytes, in order to enrich the knowledge of this type of cartilage as a cell reservoir. Auricular cartilage remnants were obtained from pediatric patients with microtia undergoing reconstructive procedures. Extracellular matrix composition was characterized using immunofluorescence and histological staining methods. Chondrocytes were isolated and expanded in vitro using a mechanical-enzymatic protocol. Chondrocyte phenotype was analyzed using qualitative PCR. Microtia cartilage preserves structural organization similar to healthy elastic cartilage. Extracellular matrix is composed of typical cartilage proteins such as type II collagen, elastin and proteoglycans. Chondrocytes displayed morphological features similar to chondrocytes derived from healthy cartilage, expressing SOX9, COL2 and ELN, thus preserving chondral phenotype. Cell viability was 94.6 % during in vitro expansion. Elastic cartilage from microtia has similar characteristics, both architectural and biochemical to healthy cartilage. We confirmed the suitability of microtia remnant as a reservoir of chondrocytes with potential to be expanded in vitro, maintaining phenotypical features and viability. Microtia remnants are an accessible source of autologous cells for auricular reconstruction using tissue engineering strategies.
机译：外耳由弹性软骨组成。小耳畸形是先天性外耳畸形，涉及大小的小幅缩小或完全缺失。组织工程的目的是基于细胞和生物材料的利用，再生可临床植入的组织和器官。来自小口眼的残留物代表使用组织工程学进行耳廓重建的细胞来源。为了检查小耳软骨的大分子结构和软骨细胞的行为，以丰富这种类型的软骨作为细胞贮藏库的知识。从接受重建手术的小口畸形儿科患者中获得耳软骨残留物。使用免疫荧光和组织学染色方法表征细胞外基质组成。分离软骨细胞，并使用机械酶联方法体外扩增。使用定性PCR分析软骨细胞表型。小耳软骨保留了类似于健康弹性软骨的结构组织。细胞外基质由典型的软骨蛋白质组成，例如II型胶原，弹性蛋白和蛋白聚糖。软骨细胞表现出与健康软骨软骨细胞相似的形态特征，表达SOX9，COL2和ELN，从而保留了软骨表型。在体外扩增期间，细胞活力为94.6％。来自小口畸形的弹性软骨在结构和生化方面均与健康软骨具有相似的特征。我们证实了小耳蜗残余物适合作为软骨细胞的贮藏库，并具有在体外扩展，保持表型特征和生存力的潜力。小耳菌残留物是使用组织工程策略进行耳廓重建的自体细胞的可获取来源。

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