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首页> 外文期刊>Cell and tissue banking: An international journal of banking, engineering & transplantation of cells and tissues >Chorionic villi derived mesenchymal like stem cells and expression of embryonic stem cells markers during long-term culturing
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Chorionic villi derived mesenchymal like stem cells and expression of embryonic stem cells markers during long-term culturing

机译：长期培养的绒毛膜绒毛间充质样干细胞及胚胎干细胞标志物的表达

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摘要

Mesenchymal stem cells (MSCs) can be obtained from a variety of human tissues. MSCs derived from placental chorionic villi of the first trimester are likely to resemble, biologically, embryonic stem cells (ESC), due to the earlier development stage of placenta. In the present study long-term cultures of MSC-like cells were assessed in order to evaluate MSCs multipotent characteristics and molecular features during the period of culture. CV-cells obtained from 10 samples of chorionic villus displayed typical fibroblastoid morphology, undergone 20 passages during a period of 120 days, maintaining a stable karyotype throughout long term expansion. The cells were positive, for CD90, CD73, CD105, CD29, CD44, HLA ABC antigens and negative for CD14, CD34, AC133, and HLA DR antigens as resulted from the flow cytometry analysis. CV-cells were differentiated in adipocytes, osteoblasts, chondrocytes and neuronal cells under specific culture conditions. The expression of the ESC-gene markers POU5F1 (Oct-4) and NANOG was observed at earliest stages (4-12 passages) and not at the late stages (14-20 passages) by RT-PCR analysis. ZFP42 and SOX2 expression were not detected. Moreover, CV-cells were found to express GATA4 but not NES (Nestin). Chorionic villi-derived cells possess multipotent properties, display high proliferation rate and self-renew capacity, share common surface antigens with adult MSCs and express certain embryonics stem cells gene markers. These characteristics highlight chorionic villi as an attractive source of MSCs for the needs of regenerative medicine.
机译：间充质干细胞（MSCs）可以从多种人体组织中获得。由于胎盘的发育较早，从头三个月的胎盘绒毛膜绒毛获得的MSCs在生物学上可能类似于胚胎干细胞（ESC）。在本研究中，对MSC样细胞的长期培养进行了评估，以评估培养期间MSC的多能性特征和分子特征。从绒毛膜绒毛的10个样品中获得的CV细胞显示出典型的成纤维细胞形态，在120天的时间内经历了20次传代，在整个长期扩增过程中保持稳定的核型。根据流式细胞术分析，细胞对CD90，CD73，CD105，CD29，CD44，HLA ABC抗原呈阳性，而对CD14，CD34，AC133和HLA DR抗原呈阴性。在特定培养条件下，CV细胞在脂肪细胞，成骨细胞，软骨细胞和神经元细胞中分化。通过RT-PCR分析，在早期（4-12代）而不是晚期（14-20代）观察到ESC基因标记POU5F1（Oct-4）和NANOG的表达。未检测到ZFP42和SOX2表达。此外，发现CV细胞表达GATA4，但不表达NES（Nestin）。绒毛膜绒毛来源的细胞具有多能性，显示出高增殖速率和自我更新能力，与成年MSC共享共同的表面抗原，并表达某些胚胎干细胞基因标记。这些特征突出了绒毛膜绒毛，作为满足再生医学需要的MSC的有吸引力的来源。

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