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首页> 外文期刊>Molecular membrane biology >Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16.
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Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16.

机译：Trans-Golgi网络语法10的功能与语法6和16的功能截然不同。

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摘要

Syntaxin 10 is a soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) protein localized to the trans-Golgi network (TGN), where two other members of the syntaxin family, syntaxins 6 and 16, also reside. The role of syntaxin 10 in regulating TGN protein traffic is not yet defined. Syntaxin 10 co-localizes well with syntaxins 6 and 16 at the TGN in interphase cells, and appears to interact with both syntaxin 6 and 16 as evidenced by co-immunoprecipitation analyses. However, unlike syntaxin 6 and 16, neither syntaxin 10 antibodies nor its cytosolic domain inhibits endosome-TGN transport of shiga toxin. Syntaxin 16 knockdown with small interfering RNA (siRNA) affects the TGN localization of syntaxin 6 but not syntaxin 10, and clearly inhibits endosome-TGN transport. On the other hand, knockdown of syntaxin 10 expressions had no significant effect on the TGN localization of syntaxin 6 and 16, and did not inhibit endosome-TGN transport. Unlike syntaxin 16, syntaxin 10 does not interact specifically with Vps45, the Sec1/Munc18 (SM) family member at the TGN. On the other hand, syntaxin 10 reciprocally co-immunoprecipitated endosomal syntaxin 12/13, and knockdown of syntaxin 10 expressions affects the surface expression of transferrin receptor (TfR) and seems to induce the formation of an immobile TfR pool. Therefore, in spite of its co-localization and possible interaction with syntaxin 16, syntaxin 10 is not part of the syntaxin 16-based SNARE complex involved in endosome-TGN transport, and may have a hitherto unrecognized function in the TGN-endosome boundary.
机译：Syntaxin 10是一种可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）蛋白，位于反高尔基网络（TGN）上，syntaxin家族的其他两个成员，syntaxin 6和16也位于其中。尚未定义syntaxin 10在调节TGN蛋白运输中的作用。在共间期细胞中，Syntaxin 10与Syntaxin 6和16很好地共定位在TGN中，并且似乎与cosminin 6和16相互作用，共同免疫沉淀分析表明。但是，与语法素6和16不同，语法素10抗体及其胞质结构域均不能抑制志贺毒素的内体TGN转运。带有小干扰RNA（siRNA）的Syntaxin 16敲低会影响Syntaxin 6的TGN定位，但不会影响Syntaxin 10的TGN定位，并明显抑制内体TGN转运。另一方面，敲低syntaxin 10表达对syntaxin 6和16的TGN定位没有显着影响，并且不抑制内体-TGN转运。与语法16不同，语法10并不专门与TGN上的Sec1 / Munc18（SM）系列成员Vps45进行交互。另一方面，syntaxin 10相互共免疫沉淀了内体syntaxin 12/13，而syntaxin 10表达的敲低会影响转铁蛋白受体（TfR）的表面表达，并似乎诱导了固定的TfR库的形成。因此，尽管其共定位并且可能与语法in 16进行交互，但是语法in 10并不是参与内体-TGN转运的基于语法in 16的SNARE复合体的一部分，并且可能在TGN-内体边界中具有迄今尚未被认识的功能。

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