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首页> 外文期刊>Science in China, series B. Chemistry,Life Sciences and Earth Sciences >Inhibitory effect of 3'-untranslated region (3'-UTR) of human tissue-plasminogen activator (ht-PA) mRNA on its expression
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Inhibitory effect of 3'-untranslated region (3'-UTR) of human tissue-plasminogen activator (ht-PA) mRNA on its expression

机译：人纤溶酶原激活物（ht-PA）mRNA 3'非翻译区（3'-UTR）对其表达的抑制作用

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摘要

A series of t-PA cDNA mutants containing different parts of 3'-UIR sequences have been constructed. In vitro translation of t-PA transcripts in rabbit reticulocyte lysates and its expression in COS-7 cells show that the 3'-UTR sequence has a very strong inhibitory effect on t-PA translation. The deletion of 3'-UTR results in 3—8-fold increase of t-PA expression. Further study shows that an AU-rich sequence of some 200 nt at 3' end of 3'-UTR is responsible for the translational inhibition, RNA stability experiment reveals that the AU-rich segment leads to a 3-fold decrease of t-PA mRNA stability. The insertion of this segment into the 3'-UTR of luciferase gene results in an obvious inhibition of Luc expression. A model is proposed for the regulationof t-PA expression.
机译：已经构建了一系列含有3'-UIR序列不同部分的t-PA cDNA突变体。兔网织红细胞裂解物中t-PA转录本的体外翻译及其在COS-7细胞中的表达表明3'-UTR序列对t-PA的翻译具有非常强的抑制作用。 3'-UTR的缺失导致t-PA表达增加3-8倍。进一步的研究表明，在3'-UTR的3'末端约200 nt的富含AU的序列引起翻译抑制; RNA稳定性实验表明，富含AU的片段导致t-PA降低了3倍mRNA稳定性。该区段插入荧光素酶基因的3'-UTR会导致对Luc表达的明显抑制。提出了调控t-PA表达的模型。

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