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首页> 外文期刊>Molecular Microbiology >Proteomic studies of diauxic lag in the differentiating prokaryote Streptomyces coelicolor reveal a regulatory network of stress-induced proteins and central metabolic enzymes
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Proteomic studies of diauxic lag in the differentiating prokaryote Streptomyces coelicolor reveal a regulatory network of stress-induced proteins and central metabolic enzymes

机译：区分原核链霉菌天蓝色链霉菌中的双体滞后的蛋白质组学研究揭示了应激诱导的蛋白质和中央代谢酶的调控网络

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Bacteria typically undergo intermittent periods of starvation and adaptation, emulated as diauxic growth in the laboratory. In association with growth arrest elicited by metabolic stress, the differentiating eubacterium Streptomyces coelicolor not only adapts its primary metabolism, but can also activate developmental programmes leading to morphogenesis and antibiotic biosynthesis. Here, we report combined proteomic and metabolomic data of S. coelicolor used to analyse global changes in gene expression during diauxic growth in a defined liquid medium. Cultures initially grew on glutamate, providing the nitrogen source and feeding carbon (as 2-oxoglutarate) into the TCA cycle, followed by a diauxic delay allowing reorientation of metabolism and a second round of growth supported by NH4+, formed during prediauxic phase, and maltose, a glycolytic substrate. Cultures finally entered stationary phase as a result of nitrogen starvation. These four physiological states had previously been defined statistically by their distinct patterns of protein synthesis and heat shock responses. Together, these data demonstrated that the rates of synthesis of heat shock proteins are determined not only by temperature increase but also by the patterns and rates of metabolic flux in certain pathways. Synthesis profiles for metabolic- and stress-induced proteins can now be interpreted by the identification of 204 spots (SWICZ database presented at http://proteom.biomed.cas.cz). Cluster analysis showed that the activity of central metabolic enzymes involved in glycolysis, the TCA cycle, starvation or proteolysis each displayed identifiable patterns of synthesis that logically underlie the metabolic state of the culture. Diauxic lag was accompanied by a structured regulatory programme involving the sequential activation of heat-, salt-, cold- and bacteriostatic antibiotic (pristinamycin I, PI)-induced stimulons. Although stress stimulons presumably provide protection during environmental- or starvation-induced stress, their identities did not reveal any coherent adaptive or developmental functions. These studies revealed interactive regulation of metabolic and stress response systems including some proteins known to support developmental programmes in S. coelicolor. [References: 60]
机译：细菌通常经历饥饿和适应的间歇期，在实验室中模拟为双生生长。伴随着代谢压力引起的生长停滞，区分性真细菌蓝细菌链霉菌不仅适应其初级代谢，而且还可以激活导致形态发生和抗生素生物合成的发育程序。在这里，我们报告结合的蛋白质组学和代谢组学数据的天蓝色链霉菌用于分析在定义的液体培养基中双生生长期间基因表达的全球变化。培养物最初在谷氨酸上生长，提供氮源并将碳（作为2-氧戊二酸）供入TCA循环，随后是双营养延迟，使代谢重新定向，并在营养前阶段和麦芽糖中形成了第二轮由NH4 +支持的生长，是糖酵解底物。由于氮缺乏，培养物最终进入静止期。这四种生理状态先前已通过其蛋白质合成和热休克反应的不同模式进行了统计学定义。总之，这些数据表明，热激蛋白的合成速率不仅取决于温度升高，还取决于某些途径中代谢通量的模式和速率。现在可以通过识别204个斑点来解释代谢和应激诱导的蛋白质的合成概况（SWICZ数据库，网址为http://proteom.biomed.cas.cz）。聚类分析表明，参与糖酵解，TCA循环，饥饿或蛋白水解的中央代谢酶的活性均显示出可识别的合成模式，这些模式在逻辑上是培养物代谢状态的基础。 Diauxic滞后伴随着一个结构化的调节程序，该程序涉及依次激活由热，盐，冷和抑菌抗生素（普里斯蒂霉素I，PI）诱导的刺激物。尽管应激刺激可能在环境或饥饿引起的应激中提供保护，但它们的身份并没有揭示出任何连贯的适应或发育功能。这些研究揭示了代谢和应激反应系统的交互调节，其中包括一些已知支持天蓝色链霉菌发育程序的蛋白质。 [参考：60]

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