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首页> 外文期刊>Scientia horticulturae >Genetic differences among 'Luotian-tianshi' (Diospyros kaki Thunb.)TI Genetic differences among 'Luotian-tianshi' (Diospyros kaki Thunb.) genotypes native to China revealed by ISSR and IRAP markers
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Genetic differences among 'Luotian-tianshi' (Diospyros kaki Thunb.)TI Genetic differences among 'Luotian-tianshi' (Diospyros kaki Thunb.) genotypes native to China revealed by ISSR and IRAP markers

机译：ISSR和IRAP标记揭示的“罗田天狮”基因型的遗传差异TI基因在中国本土的“罗田天狮”基因型中的遗传差异。

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摘要

'Luotian-tianshi' (Diospyros kaki Thunb.), which has been geographically distributed only in Dabieshan Mountain around the junction of Hubei, Henan and Anhui Province in central China, is believed to be the first PCNA (pollination-constant non-astringent) persimmon native to China. An informative scenario about the genetic diversity and phylogenetic relationships of this native PCNA cultivar is still lacking. To understand the genetic diversity, we have effectively utilized ISSR and IRAP markers to predict the genetic differences among 43 'Luotian-tianshi' genotypes collected from three major producing areas in China. A total number of 280 amplicons were observed ranging between 250 and 2000 bp using ISSR and IRAP markers. ISSR markers (100%) revealed a higher polymorphism than the IRAP markers (97.33%). On the basis of cluster analysis in the UPGMA dendrogram, we observed that in case of ISSR the genotypes were clustered into two clusters, whereas three main clusters were obtained by IRAP, indicating that 'Luotian-tianshi' genotypes existed the genetic differences and some could be potential germplasms because of the significant genetic differences, like Acc11, Acc36, Acc40 and Acc41. 'Luotian-tianshi' was a cultivar group rather than a single cultivar, and mutation and crossover might have happened in the evolution
机译：仅在中国中部湖北，河南和安徽省交界处的大别山分布的“罗田天狮”（Diospyros kaki Thunb。）被认为是第一个PCNA（持续不变的授粉的PCNA）柿子原产于中国。仍然缺乏有关这种本地PCNA品种的遗传多样性和系统发育关系的资料丰富的情况。为了了解遗传多样性，我们有效地利用了ISSR和IRAP标记来预测从中国三个主要产区收集到的43种“罗田-天狮”基因型的遗传差异。使用ISSR和IRAP标记，观察到总共280个扩增子，范围在250和2000 bp之间。 ISSR标记（100％）显示出比IRAP标记（97.33％）更高的多态性。根据UPGMA树状图的聚类分析，我们观察到在ISSR情况下，基因型聚类为两个聚类，而IRAP获得了三个主要聚类，表明“罗田-天氏”基因型存在遗传差异，有些可能由于显着的遗传差异（例如Acc11，Acc36，Acc40和Acc41）而成为潜在的种质。 “罗田-天狮”是一个品种组，而不是单个品种，进化过程中可能发生突变和杂交。

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