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首页> 外文期刊>Scientia horticulturae >Induction of natural defence accompanied by salicylic acid-dependant systemic acquired resistance in tomato seedlings in response to bioelicitors isolated from green algae
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Induction of natural defence accompanied by salicylic acid-dependant systemic acquired resistance in tomato seedlings in response to bioelicitors isolated from green algae

机译：响应自绿藻分离的生物引发剂，诱导番茄幼苗中的自然防御并伴随水杨酸依赖性全身获得性抗性

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Poly- and oligosaccharides isolated from the green algae Ulva lactuca were tested for their capacity elicitor of natural defences in tomato seedlings. After internodal infiltration of the bioelicitor solution in the midstem of tomato seedlings, the elicitor capacities were evaluated by determining the activity of phenylalanine ammonia-lyase (PAL) in the leaves located above and below the elicitation site. The results obtained showed that the crude extract of U. lactuca polysaccharides expressed an elicitor capacity of PAL activity in leaves. This expression level was similar and even higher than the ones clue to other algae polysaccharides (carrageenan, laminarin and alginate). The crude extract of polysaccharides contained two polysaccharides, the glucuronan (unsulfated homopolymer) and the ulvan (sulfated heteropolymer). The glucuronan had no significant elicitor effect whereas the ulvan induced an elicitor capacity higher than that of the crude polysaccharidic extract. The desulfation of the ulvan caused a suppression of its elicitor capacity of PAL activity. The oligoulvans (sulfated beta-Delta-(4,5)-oligorhamnoglucuronans. degree of polymerization average = 2) and the oligoglucuronans (beta-Delta-(4,5)-oligoglucuronans, degree of polymerization average = 3) were prepared from the ulvan and the glucuronan using two specific enzymes a ulvan lyase and a glucuronan lyase, purified from the bacterial strain Ochrobactrum sp. PEC2. The treatment of tomato seedlings with the oligoulvans was accompanied by a strong stimulation of PAL activity, reaching a maximum activity of 2-2.5 times higher than that of the ulvan polymer. Although the oligoglucuronans expressed an elicitor capacity in PAL activity twice higher than that of the glucuronan polymer, its induction was less important than the oligoulvans. The high elicitor capacity of these saccharides seemed to be related to sulfate substituents and the rhamnose residues. The stimulation of PAL activity was accompanied by an increase of phenolic compounds content and an induction of salicylic acid in the leaves located above and below the elicitation site. The treatments of tomato seedlings with U. lactuca elicitors significantly reduced wilt development caused by Fusarium oxysporum f. sp. lycopersici. The percent of wilting and mortality after 45 days was markedly reduced when plants were treated with oligoulvans, respectively 44% and 54% compared to the control plants. The whole of these results showed clearly those U. lactuca elicitors, particularly the ulvan and the oligoulvans, induced in tomato seedlings the stimulation of natural systemic defences accompanied by a systemic acquired resistance that seems to be salicylic acid-dependent. (C) 2012 Elsevier By
机译：测试了从绿藻Ulva lactuca分离出的多糖和寡糖的番茄幼苗中自然防御能力的诱发因素。在番茄幼苗中部的生物激发子溶液发生节间渗透后，通过测定激发点上方和下方的叶片中苯丙氨酸氨解酶（PAL）的活性来评估激发子容量。所获得的结果表明，乳酸链球菌多糖的粗提物在叶片中表达了激发PAL活性的能力。该表达水平与其他藻类多糖（角叉菜胶，层粘连蛋白和藻酸盐）相似，甚至更高。多糖的粗提物含有两种多糖，葡糖醛酸聚糖（未硫酸化的均聚物）和ulvan（硫酸化的杂聚物）。葡糖醛酸聚糖没有显着的激发子作用，而ulvan诱导的激发子能力高于粗多糖提取物。上皮的脱硫导致其PAL活性的引发能力受到抑制。寡聚葡糖（硫酸化的β-δ-（4,5）-寡聚葡糖醛酸。聚合度平均值= 2）和寡葡糖醛酸（β-δ-（4,5）-寡聚葡糖醛酸，聚合度= 3）是由ulvan和lucuronannan使用两种特异性酶ulvan裂合酶和glucouronan裂合酶，从细菌菌株Ochrobactrum sp。纯化。 PEC2。用低聚果糖处理番茄幼苗伴随着PAL活性的强烈刺激，其最大活性比ulvan聚合物高2-2.5倍。尽管寡葡糖醛酸聚糖在PAL活性中表达的诱导子能力是葡糖醛酸聚糖聚合物的两倍，但其诱导作用却比寡聚葡糖少。这些糖的高激发子能力似乎与硫酸盐取代基和鼠李糖残基有关。 PAL活性的刺激伴随着酚类化合物含量的增加和水杨酸在诱导位点上方和下方的叶片中的诱导。用U. lactuca引发剂处理番茄幼苗可显着降低尖孢镰刀菌引起的枯萎。 sp。 lycopersici。用低聚果糖处理植物后，45天后的萎百分率和死亡率显着降低，与对照植物相比分别降低了44％和54％。所有这些结果清楚地表明，那些U. lactuca引发剂，尤其是ulvan和oligoulvans，在番茄幼苗中诱导了对天然系统防御的刺激，并伴随着系统获得性抗性，似乎是水杨酸依赖性的。 （C）2012爱思唯尔

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