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首页> 外文期刊>Molecular Nutrition and Food Research >Quantification of free and protein-bound trans-resveratrol metabolites and identification of trans-resveratrol-C/O-conjugated diglucuronides - Two novel resveratrol metabolites in human plasma
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Quantification of free and protein-bound trans-resveratrol metabolites and identification of trans-resveratrol-C/O-conjugated diglucuronides - Two novel resveratrol metabolites in human plasma

机译：游离和结合蛋白质的反式白藜芦醇代谢产物的定量和反式白藜芦醇-C / O-缀合的葡糖醛酸苷的鉴定-人血浆中的两种新型白藜芦醇代谢物

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摘要

The polyphenol trans-resveratrol (t-RES) is present as t-RES-3-O-o-D-glycoside, termed piceid, in several plant-derived foods. Although data on the metabolism and on in vivo effects of t-RES have been reported, quantitative data on the metabolites formed after dietary intake of t-RES or piceid are still lacking. In this study, 85.5 mg of piceid per 70 kg of body weight (bw) were administered to healthy volunteers in a bolus dose. t-RES metabolites formed in plasma and urine were identified and quantified by LC-MS/MS, NMR, and HPLC-DAD analysis using chemically synthesized t-RES conjugate standards. In addition, the amount of t-RES metabolites bound noncovalently to plasma proteins was determined for the first time in humans. The metabolites identified and quantified were t-RES-3-sulfate, t-RES-3,4'-disulfate, t-RES-3,5-disulfate, t-RES-3-glucuronide and t-RES-4'-glucuronide, with t-RES-sulfates being the dominant conjugates in plasma and urine. Besides these metabolites, two novel t-RES-C/O-conjugated diglucuronides have been identified and quantified in plasma and urine. Moreover, it could be shown that up to 50% of the plasma t-RES-3-sulfate, t-RES-disulfates, and the novel t-RES-C/O-diglucuronides were bound to proteins. Total recovery of the dietary administered piceid in urine ranged between 13.6 and 35.7%.
机译：多酚反式白藜芦醇（t-RES）在几种植物来源的食品中以t-RES-3-O-o-D-糖苷的形式存在，被称为piceid。尽管已经报道了关于t-RES的代谢和体内作用的数据，但是仍然缺乏关于从饮食中摄取t-RES或木虱后形成的代谢物的定量数据。在这项研究中，每70公斤体重（bw）服用85.5毫克的木素类药物，以大剂量注射给健康志愿者。使用化学合成的t-RES共轭标准物通过LC-MS / MS，NMR和HPLC-DAD分析鉴定和定量血浆和尿液中形成的t-RES代谢物。此外，首次在人类中确定了与血浆蛋白非共价结合的t-RES代谢物的量。鉴定和定量的代谢产物是t-RES-3-硫酸盐，t-RES-3,4'-二硫酸盐，t-RES-3,5-二硫酸盐，t-RES-3-葡萄糖醛酸和t-RES-4'-葡萄糖醛酸，其中t-RES-硫酸盐是血浆和尿液中的主要结合物。除了这些代谢物，在血浆和尿液中还鉴定和定量了两种新型的t-RES-C / O-缀合的葡糖醛酸苷。此外，可以证明血浆中最多有50％的t-RES-3-硫酸盐，t-RES-二硫酸盐和新型t-RES-C / O-葡糖醛酸苷与蛋白质结合。尿中饮食中施用的糊精的总回收率介于13.6和35.7％之间。

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