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Inter- and intra-specific genetic diversity among cyclamen accessions investigated by RAPD markers

机译：RAPD标记研究仙客来种质间的种内和种内遗传多样性

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摘要

Random amplified polymorphic DNA (RAPD) analysis was used to characterize genetic diversity of 26 Cyclamen persicum and Cyclamen com accessions. Eighty-four arbitrary primers tested, among which nine primers showed reliable polymorphic banding patterns and yielded 104 polymorphic markers. Jaccard's similarity coefficient among accessions ranged from 0.99 to 0.08. At a similarity of 68%, accessions were divided into three clusters. The cophenetic correlation coefficient between the similarity matrix and the dendrogram was relatively high (r =0.9) showing the goodness of fit of the dendrogram. The RAPD analysis offered a rapid and reliable tool for the estimation of inter- and intra-specific variability in cyclamens. The wide genetic variation observed for cyclamens within Iran guarantees a promising future of breeding.
机译：随机扩增多态性DNA（RAPD）分析用于表征26个仙客来和仙客来组合的遗传多样性。测试了84个任意引物，其中9个引物显示出可靠的多态性条带模式，并产生了104个多态性标记。 Jaccard的种质相似系数在0.99到0.08之间。相似性为68％，将种质分为三类。相似度矩阵与树状图之间的显着相关系数较高（r = 0.9），显示出树状图拟合的良好性。 RAPD分析为估算仙客来种间和种内变异提供了一种快速而可靠的工具。在伊朗境内发现的仙客来的广泛遗传变异保证了有希望的育种前景。

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