...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Scientia horticulturae >BTH-mediated antioxidant system responses in apple leaf tissues
【24h】

BTH-mediated antioxidant system responses in apple leaf tissues

机译：BTH介导的苹果叶片组织抗氧化系统反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BTH (S-methylbenzo-1,2,3-thiadiazole-7-carbothiate), an active compound of the commercial preparation Bion, has been studied as an elicitor of resistance to fire blight (Erwinia amylovora) in apple. However, the biochemical mechanisms of its action are not fully elucidated. Our study indicated that BTH at the best time of its protection activity (2-14 days after application) induced changes in prooxidant-antioxidant balance in the leaves of apple trees, but in different ways in the enzymatic and nonenzymatic antioxidants. Glutathione as low molecular antioxidant as well as superoxide anion radical and lipid peroxides as oxidants exhibited changes at the early phase of BTH action. Glutathione-dependent enzymes were strongly affected by the elicitor used. On the 2nd day glutathione transferase (GST) and glutathione peroxidase (GSH-Px) activities increased by about 70% and 30% above the control, respectively. GST activity normalized about the 14th day but GSH-Px at the same time showed 27% of the control value. Among enzymes utilising hydrogen peroxide only catalase showed increase (37%) at the early phase of experiment. Compared with the control, BTH-treated plants did not show changes in ascorbate peroxidase and phenylalanine ammonia-lyase activities. Tocopherol (TOC) level diminished starting from the 7th day after BTH treatment and on the 14th day it was only 28% of the control. It is proposed that extinguishing of BTH-mediated signal resulted from TOC and glutathione action. The diminished ascorbate level at all examined times may play a crucial role in BTH-mediated cell growth regulation. The direct influence of BTH on lipid metabolism should be also taken into consideration.
机译：BTH（S-甲基苯并-1,2,3-噻二唑-7-碳甲酸酯），一种商业化制剂Bion的活性化合物，已被研究作为引起苹果抗白叶枯病（Erwinia amylovora）的诱因。但是，其作用的生化机制尚未完全阐明。我们的研究表明，BTH在其保护活性的最佳时间（施用后2-14天）诱导了苹果树叶片中抗氧化剂-抗氧化剂平衡的变化，但在酶促抗氧化剂和非酶促抗氧化剂中的变化方式不同。谷胱甘肽作为低分子抗氧化剂以及超氧阴离子自由基和脂质过氧化物作为氧化剂在BTH作用的早期阶段表现出变化。谷胱甘肽依赖性酶受到所用激发子的强烈影响。在第2天，谷胱甘肽转移酶（GST）和谷胱甘肽过氧化物酶（GSH-Px）活性分别比对照增加约70％和30％。 GST活性在第14天左右恢复正常，但同时GSH-Px显示出对照值的27％。在利用过氧化氢的酶中，只有过氧化氢酶在实验的早期显示出增加（37％）。与对照相比，经BTH处理的植物在抗坏血酸过氧化物酶和苯丙氨酸氨解酶活性方面没有变化。从BTH治疗后的第7天开始，生育酚（TOC）的水平开始降低，而在第14天，生育酚的水平仅为对照的28％。有人提出，由TOC和谷胱甘肽作用导致BTH介导信号的熄灭。在所有检查时间中，抗坏血酸水平的降低可能在BTH介导的细胞生长调节中起关键作用。还应考虑BTH对脂质代谢的直接影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号