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首页> 外文期刊>Cerebral cortex >Altered spatial arrangement of layer V pyramidal cells in the mouse brain following prenatal low-dose X-irradiation. A stereological study using a novel three-dimensional analysis method to estimate the nearest neighbor distance distributions of cell
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Altered spatial arrangement of layer V pyramidal cells in the mouse brain following prenatal low-dose X-irradiation. A stereological study using a novel three-dimensional analysis method to estimate the nearest neighbor distance distributions of cell

机译：产前低剂量X射线照射后，小鼠大脑中V层锥体细胞的空间排列发生了变化。使用新型三维分析方法估算单元格最近邻距离分布的立体研究

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摘要

Prenatal X-irradiation, even at doses <1 Gy, can induce spatial disarray of neurons in the brains of offspring, possibly due to disturbed neuronal migration. Here we analyze the effects of prenatal low-dose X-irradiation using a novel stereological method designed to investigate the three-dimensional (3D) spatial arrangement of neurons in thick sections. Pregnant mice were X-irradiated with 50 cGy on embryonic day 13 or were sham-irradiated. The right brain halves of their 180-day-old offspring were dissected into entire series of 150 microm thick frontal cryostat sections and stained with gallocyanin. Approximately 700 layer V pyramidal cells per animal were sampled in a systematic-random manner in the middle of the section's thickness. The x-y-z coordinates of these 'parent neurons' were recorded, as well as of all neighboring (up to 10) 'offspring neurons' close to each 'parent neuron'. From these data, the nearest neighbor distance (NND) distributions for layer V pyramidal cells were calculated. Using this novel 3D analysis method, we found that, in comparison to controls, prenatal X-irradiation had no effect on the total neuron number, but did cause a reduction in the mean volume of layer V by 26.5% and a more dispersed spatial arrangement of these neurons. Considering the recent literature, it seems reasonable to consider abnormal neuronal migration as the potential basic cause of this finding.
机译：产前X射线辐射，即使剂量<1 Gy，也可能导致后代大脑中神经元的空间混乱，这可能是由于神经元迁移受阻所致。在这里，我们使用一种新颖的立体学方法来分析产前低剂量X射线的影响，该方法旨在研究厚部分中神经元的三维（3D）空间排列。怀孕的小鼠在胚胎第13天进行了50 cGy的X射线辐照或假辐照。将其180天大的后代的右半脑解剖成整个150微米厚的前低温恒温器切片系列，并用花青素染色。以系统随机的方式在切片厚度的中间对每只动物采样约700层V锥体细胞。记录这些“父神经元”以及每个“父神经元”附近的所有相邻（最多10个）“后代神经元”的x-y-z坐标。根据这些数据，计算出V层锥体细胞的最近邻距离（NND）分布。使用这种新颖的3D分析方法，我们发现，与对照组相比，产前X射线辐照对总神经元数没有影响，但确实使V层的平均体积减少了26.5％，并且空间分布更加分散这些神经元。考虑到最近的文献，将异常的神经元迁移视为这一发现的潜在基本原因似乎是合理的。

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