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首页> 外文期刊>Biomaterials >Inflammatory signals in the development of tissue-engineered soft tissue.
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Inflammatory signals in the development of tissue-engineered soft tissue.

机译:组织工程性软组织发育中的炎症信号。

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摘要

Scaffolds with 400mum pores constructed from hyaluronan modified by benzyl esterification of the carboxylic acid groups (HYAFF-11) and viscous gels created from dodecyl-amidation of hyaluronan (HYADD-3) were implanted subcutaneously into rats for periods of up to 26 and 12 weeks, respectively. Tissue explants were infiltrated with methacrylate resin, sectioned and stained with a broad panel of inflammatory markers in addition to conventional histological stains. Both gels and sponges became rapidly infiltrated by cells that, in the case of HYAFF sponges, did not differentiate, whilst mature adipocytes were only observed at the margins of the sponges. This was combined with sustained inflammatory antigen expression. Conversely, in the HYADD gels, only moderate inflammatory staining was observed at 4 weeks which had diminished completely by 8 weeks. Mature and maturing adipocytes were observed deep within the gels. It is hypothesised that the gels present an excellent inflammatory cytokine profile which induces macrophage infiltration, proliferation then differentiation into adipocytes and is responsible for the generation of neoadipogenesis.
机译:将透明质酸经羧甲基苄基酯化修饰的透明质酸构建的支架(HYAFF-11)和具有400个孔的支架和透明质酸的十二烷基酰胺化形成的粘性凝胶(HYADD-3)皮下植入大鼠中长达26周和12周, 分别。组织外植体用甲基丙烯酸酯树脂浸润,切成薄片并用除常规组织学染色剂外的各种炎症标记物染色。凝胶和海绵都迅速被细胞浸润,对于HYAFF海绵,它们没有分化,而成熟的脂肪细胞仅在海绵的边缘观察到。这与持续的炎性抗原表达结合。相反,在HYADD凝胶中,在第4周仅观察到中等程度的炎症染色,而在第8周时完全消失。在凝胶深处观察到成熟和成熟的脂肪细胞。假设凝胶具有优异的炎性细胞因子特征,其诱导巨噬细胞浸润,增殖然后分化成脂肪细胞,并负责新脂肪形成的产生。

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