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首页> 外文期刊>Scandinavian journal of immunology. >Identification of Rv2041c, a novel immunogenic antigen from Mycobacterium tuberculosis with serodiagnostic potential.
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Identification of Rv2041c, a novel immunogenic antigen from Mycobacterium tuberculosis with serodiagnostic potential.

机译：Rv2041c的鉴定，一种来自结核分枝杆菌的具有血清诊断潜力的新型免疫原性抗原。

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Novel immunogenic antigens are continually required for the improvement of diagnostic techniques for Mycobacterium tuberculosis infection. Some proteins with serodiagnostic value are not expressed under normal culture conditions, but may be induced under specific conditions such as gradual oxygen depletion and low pH, and from inside macrophages. Using a customized amplification library, we previously found that Rv2041c from M. tuberculosis H37Rv was highly expressed in vitro under conditions of low pH and hypoxia. In this study, recombinant (r)Rv2041c was produced in Escherichia coli to examine its role in immune responses. Increased Rv2041c expression in vitro during dormancy and during infection in human macrophages was confirmed by Western blotting and reverse transcription polymerase chain reaction, respectively. Interestingly, positive antibody responses to rRv2041c were detected only in those patients with active tuberculosis (TB) and in mice infected with M. tuberculosis H37Rv. Finally, Rv2041c was used successfully in the serodiagnosis of active M. tuberculosis infection in Korean patients in conjunction with other M. tuberculosis proteins, including Ag85 complex, 38 kDa, rESAT-6, rHSP-X and rCFP-10. Our Rv2041c-ELISA had comparable diagnostic sensitivity and equivalent specificity to the use of an M. tuberculosis H37Rv cellular extract. In addition, seven of 46 serum samples collected from TB patients (15.28%) showed positive antibody responses to Rv2041c, but not to the other proteins. These results suggest that Rv2041c can be used to increase assay sensitivity alongside well-known antigens for the serodiagnosis of M. tuberculosis infection.
机译：不断需要新型的免疫原性抗原来改善结核分枝杆菌感染的诊断技术。某些具有血清学诊断价值的蛋白质在正常培养条件下不表达，但可能在特定条件下被诱导，例如逐渐耗氧和低pH值，以及从巨噬细胞内部被诱导。使用定制的扩增文库，我们先前发现来自结核分枝杆菌H37Rv的Rv2041c在低pH和缺氧条件下在体外高表达。在这项研究中，重组（r）Rv2041c在大肠杆菌中产生，以检查其在免疫应答中的作用。分别通过蛋白质印迹和逆转录聚合酶链反应证实了在人类巨噬细胞休眠期间和感染期间体外Rv2041c表达的增加。有趣的是，仅在那些患有活动性结核病（TB）的患者和感染了结核分枝杆菌H37Rv的小鼠中检测到对rRv2041c的阳性抗体反应。最后，Rv2041c与其他结核分枝杆菌蛋白，包括Ag85复合物，38 kDa，rESAT-6，rHSP-X和rCFP-10一起，成功用于韩国患者的活动性结核分枝杆菌感染的血清学诊断。我们的Rv2041c-ELISA具有与结核分枝杆菌H37Rv细胞提取物相当的诊断灵敏度和同等的特异性。此外，从结核病患者收集的46份血清样品中有7份（15.28％）对Rv2041c呈阳性抗体反应，但对其他蛋白质无阳性反应。这些结果表明，Rv2041c可与已知抗原一起用于结核分枝杆菌感染的血清诊断，以提高检测灵敏度。

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