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首页> 外文期刊>Scandinavian Journal of Laboratory Animal Science >Blood Sampling from the Tail Vein, in Comparison with Two Other Techniques, Causes Less Stress to Mice
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Blood Sampling from the Tail Vein, in Comparison with Two Other Techniques, Causes Less Stress to Mice

机译：与其他两种技术相比，从尾静脉进行采血可减少对小鼠的压力

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It is important to use the optimal method for repeated blood sampling to ensure minimal stress to mice, and also to provide better pharmacokinetic and pharmacodynamic data. The aim of the present study was to compare the impact of blood sampling methods on corticosterone and adrenocorticotropic hormone (ACTH) levels in mice.Hsdwin:NMRI mice were divided into four sampling groups: control group (1), vena facialis (11), tail vein (111) and saphenous vein (IV). The first blood samples, obtained from vena facialis, tail or saphenous vein of conscious mice, were taken at time point 0. The second blood sample was taken by decapitation from groups II-IV with isoflurane anaesthesia at time point 20 min. The control group animals were anesthetized and decapitated at 20 min time point. Corticosterone levels in plasma were analyzed at time point 0 and 20 min, and ACTH at time point 20 min.Saphenous bled mice, in comparison with vena facialis and tail vein sampled mice, indicated statistically significant greater (P < 0.05) level of corticosterone at sampling point (0 min). Rising levels of corticosterone in all groups differed statistically (P < 0.05) from the control group level, indicating that all tested bleeding methods were stressful to the experimental animals. However, the tail vein bleeding method stressed statistically significantly (P < 0.05) less in comparison with vena facialis and saphenous vein bleeding. At time point 20 min, only saphenous vein bled mice showed statistically significant greater (P < 0.05) blood levels of ACTH compared to tail vein bled mice.Conditions in sampling and rising levels of corticosterone and/or ACTH level did not show direct correlation. In conclusion the results suggest that the tail bleeding method accomplished least stress to mice and next less vena facialis bleeding. Blood collection technique from the saphenous vein was the most stressful to the experimental animals.
机译：重要的是使用最佳方法进行重复血液采样，以确保对小鼠的压力最小，并提供更好的药代动力学和药效学数据。本研究的目的是比较采血方法对小鼠皮质酮和促肾上腺皮质激素（ACTH）水平的影响。Hsdwin：NMRI小鼠分为四个采样组：对照组（1），面静脉（11），尾静脉（111）和隐静脉（IV）。在时间点0采集从清醒小鼠的面静脉，尾巴或隐静脉获得的第一份血样。在第20分钟的时间点用异氟烷麻醉从II-IV组断头取第二份血样。对照组动物在20分钟时间点麻醉并断头。在0和20分钟的时间点分析血浆皮质酮水平，在20分钟的时间点分析促肾上腺皮质激素。与面静脉和尾静脉采样的小鼠相比，隐性出血小鼠的皮质酮水平在统计学上更高（P <0.05）采样点（0分钟）。所有组中皮质酮的升高水平与对照组水平有统计学差异（P <0.05），这表明所有测试的出血方法对实验动物产生压力。然而，与面静脉和大隐静脉出血相比，尾静脉出血方法的压力显着降低（P <0.05）。在20分钟的时间点，只有隐性静脉出血小鼠的ACTH血液水平显着高于尾静脉血液小鼠（P <0.05）。皮质酮和/或ACTH水平的采样和升高条件没有显示直接相关性。总之，结果表明，尾巴出血方法对小鼠的压力最小，其次是面部面部静脉出血。从隐静脉收集血液对实验动物的压力最大。

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