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首页> 外文期刊>Biomaterials >MORPHOLOGICAL EVALUATION OF OSTEOBLASTS CULTURED ON DIFFERENT CALCIUM PHOSPHATE CERAMICS
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MORPHOLOGICAL EVALUATION OF OSTEOBLASTS CULTURED ON DIFFERENT CALCIUM PHOSPHATE CERAMICS

机译：在不同磷酸钙陶瓷上培养的成骨细胞的形态学评价

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The objective of these investigations was to develop an in vitro test system for evaluating novel rapidly resorbable calcium phosphate ceramics of varying composition. Fat bone marrow cells were cultured on the disc-shaped test substrates for 14 days. Five calcium phosphates were examined: R1, CaNaPO4; R1/M2, composed of CaNaPO4 and MgNaPO4; R1/2 composed of CaNaPO4 and MS2SiO4; R1+9% SiO2 consisting of CaNaPO4 and 9% SiO2 (wt%) and R17, Ca2KNa(PO4)(2). Two studies were performed. In study I cultures were re-fed every two to three days. In study II the medium was changed daily, and calcium and phosphate concentrations of the medium were determined daily. Specimens were prepared for light microscopy and morphometric evaluation of the cell-covered substrate area, scanning electron microscopy and energy-dispersive X-ray analysis. With all materials tested except for R1/2, an increase of cellular growth was observed after changing the medium daily. Of the different calcium phosphate ceramics tested, R17 and R1/M2 facilitated osteoblast growth and elaboration of extracellular matrix to the highest degree. The inhibition of cell growth encountered with R1 in study I and R1/2 in both studies seemed to be related to a high phosphate-ion release from these materials. (C) 1997 Elsevier Science Limited. All rights reserved. [References: 28]
机译：这些研究的目的是开发一种体外测试系统，用于评估各种组成的新型可快速吸收的磷酸钙陶瓷。脂肪骨髓细胞在圆盘形的测试基质上培养14天。检查了五种磷酸钙：R1，CaNaPO4; R1 / M2，由CaNaPO4和MgNaPO4组成; R1 / 2由CaNaPO4和MS2SiO4组成;由CaNaPO4和9％SiO2（wt％）组成的R1 + 9％SiO2和R17，Ca2KNa（PO4）（2）。进行了两项研究。在研究中，我每两到三天重新喂一次文化。在研究II中，每天更换培养基，并每天测定培养基中钙和磷酸盐的浓度。制备样品用于光学显微镜和细胞覆盖的基质区域的形态测定，扫描电子显微镜和能量色散X射线分析。对于除R1 / 2以外的所有测试材料，每天更换培养基后观察到细胞生长的增加。在测试的不同磷酸钙陶瓷中，R17和R1 / M2在最大程度上促进了成骨细胞的生长和细胞外基质的形成。在两项研究中，研究I和R1 / 2中R1遇到的细胞生长抑制作用似乎与这些物质中高的磷酸根离子释放有关。 （C）1997 Elsevier Science Limited。版权所有。 [参考：28]

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