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首页> 外文期刊>Seed Science and Technology >Use of SSR markers to determine the genetic purity of a popular Australian wheat variety and consequences for stripe rust reactions.
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Use of SSR markers to determine the genetic purity of a popular Australian wheat variety and consequences for stripe rust reactions.

机译：使用SSR标记确定澳大利亚流行小麦品种的遗传纯度以及条锈反应的后果。

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摘要

Sunvale is a popular bread wheat variety grown throughout central and northern New South Wales in Australia which has moderate resistance to the fungal leaf disease stripe rust. However, significant levels of stripe rust have been reported in Sunvale crops over the past few seasons. Sunvale is a 17-year-old variety so genetic purity may also be involved in the numerous reports of increased disease susceptibility in grower crops. Twelve SSR markers were used to determine the genetic purity of 23 grower-retained seed lots of cv. Sunvale. Sixteen of the 23 grower-retained Sunvale seed lots (70%) contained at least 5% contamination from a different genotype, while seven samples (30%) contained >10% contamination. The SSR analysis was complemented by field observations of the various seed lots in a replicated small plot trial. In 17 of the 19 grower-retained Sunvale seed lots that had some level of contamination with another genotype(s), at least one contaminant was very susceptible to stripe rust. The pure Sunvale plants in the plots showed a moderately resistant reaction. This is the first report of seed impurity being definitively determined as the cause of unexpected stripe rust responses within a wheat variety.
机译：Sunvale是一种流行的面包小麦品种，在澳大利亚新南威尔士州中部和北部生长，对真菌性叶病条纹锈病具有中等抵抗力。然而，在过去的几个季节中，据报道桑瓦尔的作物中出现了明显的条纹锈病。 Sunvale是一个已有17年历史的变种，因此遗传纯度可能也参与了许多关于农作物疾病易感性增加的报道。使用十二个SSR标记来确定23个种植者保留的cv种子批次的遗传纯度。桑维尔在23个种植者保留的Sunvale种子批次中，有16个（70％）至少含有5％来自不同基因型的污染，而七个样品（30％）则含有大于10％的污染。在重复的小样田试验中，通过对各种种子批次进行现场观察，对SSR分析进行了补充。在19个种植者保留的Sunvale种子批次中，有17个具有某种程度的另一种基因型污染，其中至少一种污染物非常容易发生条锈病。地块上的纯Sunvale植物表现出中等抗性。这是首次确定种子杂质是小麦品种中出现意外条锈反应的原因的第一份报告。

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