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首页> 外文期刊>Seed Science and Technology >A novel fluorescent dual-labeling method for anti-counterfeiting pelleted tobacco seeds.
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A novel fluorescent dual-labeling method for anti-counterfeiting pelleted tobacco seeds.

机译：一种新型的荧光双标记方法，用于防伪造粒的烟草种子。

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摘要

A novel fluorescent dual-labeling method with fluorescein (FR) and rhodamine B (RB) was used for anticounterfeiting pelleted seeds of tobacco (Nicotiana tabacum L.) cv. MS Yunyan85 (MSYY85) and Honghua Dajinyuan (HHDJY). Seeds were pelleted using a layer of bentonite, which was sprayed with 2.0 mg ml-1 solution RB, followed by application of FR in a second layer at a rate of 1 g per 10 g of naked seeds mixed with talc. Control seed were pelleted using bentonite sprayed with water before the talc layer was added. These two fluorescent compounds had no negative effects on the germination and seedling growth of pelleted seeds, and even significantly enhanced seedling dry weight, POD activity and contents of chlorophyll and carotenoid in MS YY85 and increased shoot height and chlorophyll content in HHDJY as compared with the control. In addition, the cotyledon tip represented bright green fluorescence under illumination of blue light (495 nm) due to FR, and meanwhile, the cotyledon vein of the same seedling showed obvious red fluorescence under green light (546 nm) based on the RB. Thus, it indicated that the new dual-labeling method that RB and FR simultaneously applied in the same seed pellets will improve anti-counterfeiting technology and enhance seed security.
机译：一种新颖的荧光双标记方法，用荧光素（FR）和若丹明B（RB）对烟草（Nicotiana tabacum L.）cv的颗粒状颗粒进行防伪。 MS Yunyan85（MSYY85）和Honghua Dajinyuan（HHDJY）。使用膨润土层将种子制成颗粒，然后用2.0 mg ml -1 RB溶液喷洒膨润土，然后在第二层中以每10 g裸露种子1 g的比例施用FR与滑石粉。在添加滑石粉之前，使用喷有水的膨润土将对照种子制粒。两种荧光化合物对颗粒种子的发芽和幼苗生长没有负面影响，甚至与MS YY85相比显着增强了MS YY85的幼苗干重，POD活性和叶绿素和类胡萝卜素的含量，并增加了HHDJY的苗高和叶绿素的含量。控制。另外，子叶尖端在蓝光（495 nm）的照射下，由于FR而表现出亮绿色荧光，同时，同一幼苗的子叶静脉在基于RB的绿光（546 nm）下显示出明显的红色荧光。因此，这表明RB和FR同时应用于同一种子颗粒中的新的双标记方法将改善防伪技术并增强种子安全性。

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