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首页> 外文期刊>Seed Science and Technology >Use of EST-SSR markers for genetic characterisation of commercial watermelon varieties and hybrid seed purity testing.
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Use of EST-SSR markers for genetic characterisation of commercial watermelon varieties and hybrid seed purity testing.

机译:EST-SSR标记用于商业西瓜品种的遗传表征和杂交种子纯度测试。

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摘要

EST-SSR markers were used to identify 36 candidate and registration watermelon varieties and 20 major commercial watermelon varieties, and to assess hybrid seed purity of a commercial hybrid watermelon variety known as 'Speedggul'. A set of 300 EST-SSR primer pairs were applied for fingerprinting the watermelon varieties. Nine markers showed hyper-variability and were able to differentiate 56 genotypes, representing the genetic variation within varietal groups. Cluster analysis and Jaccard's distance coefficients using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) categorised varieties into two major groups corresponding to morphological traits. To evaluate the use of EST-SSR marker analysis for seed purity testing of F1 hybrid watermelon Speedggul, the results of marker analysis were compared with the results of a grow-out test. The F1 purity through grow-out test was in accordance with molecular marker analysis. These markers will be useful for fingerprinting watermelon varieties and help private seed companies to improve watermelon seed purity.
机译:EST-SSR标记用于鉴定36个候选和注册西瓜品种和20个主要商品西瓜品种,并评估称为“ Speedggul”的商品杂交西瓜品种的杂交种子纯度。一组300个EST-SSR引物对用于指纹西瓜品种。九个标记物显示出高变异性,并能够区分56个基因型,代表了不同品种组内的遗传变异。使用UPGMA(算术平均值非加权对群方法)进行聚类分析和Jaccard距离系数将品种分为与形态性状相对应的两个主要组。为了评估EST-SSR标记分析在F 1 杂种西瓜Speedggul种子纯度测试中的应用,将标记分析的结果与成年试验的结果进行了比较。生长试验测得的F 1 纯度与分子标记分析结果一致。这些标记将有助于对西瓜品种进行指纹识别,并帮助私人种子公司提高西瓜种子的纯度。

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