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Matriconditioning improves the quality and protein level of medium vigor hot pepper seed

机译：基质处理可改善中等活力辣椒种子的质量和蛋白质水平

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摘要

The objective of this study was to invigorate medium vigor hot pepper seed using matriconditioning. In one experiment, medium (80 percent germination, 2 year-old) vigor seeds of hot pepper were subjected to various invigoration treatments. In the subsequent experiment, medium and high (95 percent germination, 1 year-old) vigor hot pepper seeds were subjected to matriconditioning treatment at 15 deg C for 6 days using sawdust (210 #mu# particle size) moistened with 100 #mu#M GA_3. In the later experiment, the ratio of seed to sawdust to GA_3 solution in the matriconditioning treatment was 1:2:5. Seed quality of matriconditioned medium vigor seed was enhanced over the untreated seed as indicated by a higher percent of germination, improved vigor index, and speed of germination. The total protein isolated from matriconditioned seed was 16 percent higher than that from untreated. Meanwhile, total protein isolated from high vigor seed was 10 percent higher than that from medium vigor seed. Qualitativechanges in the profiles of total protein isolated from matriconditioned seeds occurred within the 76, 45, 38, 30 and 20 kDa polypeptides. Matriconditioning with sawdust and GA_3 offers an alternative for improving seed quality of hot pepper seeds.
机译：这项研究的目的是使用基质调节来增强中等活力的辣椒种子。在一个实验中，对中势（发芽率为80％，2岁）的辣椒种子进行了各种强化处理。在随后的实验中，使用浸有100＃mu＃的木屑（粒度为210＃mu＃）在15摄氏度下对中等和高度（95％萌发，1岁）萌发的辣椒种子进行基质处理6天。 M GA_3。在随后的实验中，基质处理中种子与木屑与GA_3溶液的比例为1：2：5。经改良的中等活力种子的种子质量比未处理的种子有所提高，这可以通过较高的发芽率，改善的活力指数和发芽速度来表明。从基质改良种子中分离的总蛋白质比未处理的蛋白质高16％。同时，从高活力种子中分离出的总蛋白质比从中活力种子中分离出的总蛋白质高10％。从基质修饰的种子中分离的总蛋白质的性质发生质变，发生在76、45、38、30和20 kDa多肽内。用木屑和GA_3进行基质处理为提高辣椒种子的种子质量提供了另一种选择。

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