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首页> 外文期刊>Seed Technology >Identification of annual and perennial ryegrass using reversed-phase high-performance liquid chromatography
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Identification of annual and perennial ryegrass using reversed-phase high-performance liquid chromatography

机译：反相高效液相色谱法鉴定一年生和多年生黑麦草

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Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) of cereal grain proteins has received much attention in recent years as a quick and efficient means of cultivar identification. A study of perennial ryegrass (Lolium perenne L.) and annual ryegrass (Lolium multiflorum Lam.) was undertaken to determine if RP-HPLC could be adapted to the identification of these species. Chromatograms of different cultivars of the two species studied all had distinct peak patterns, which correlated with each kind. Protein analysis of single seeds accurately differentiated annual ryegrass seeds from perennial ryegrass seeds. Mixtures of ryegrass seeds were analyzed and compared. A lower limit of 10 percent annual ryegrass in perennial ryegrass was detectedby monitoring changes of area under the peaks of the chromatograms. RP-HPLC was shown to be a quick, repeatable and reliable method of ryegrass species identification for a general screening of seed lots.
机译：谷物蛋白质的反相高效液相色谱（RP-HPLC）作为一种快速，有效的品种鉴定手段近年来受到了广泛关注。进行了多年生黑麦草（Lolium perenne L.）和一年生黑麦草（Lolium multiflorum Lam。）的研究，以确定RP-HPLC是否适合于鉴定这些物种。所研究的两个物种的不同品种的色谱图均具有不同的峰模式，且与每种类型相关。单个种子的蛋白质分析可将多年生黑麦草种子与一年生黑麦草种子准确区分开。分析和比较了黑麦草种子的混合物。通过监测色谱图峰下面积的变化，检测到多年生黑麦草中年黑麦草的下限为10％。 RP-HPLC被证明是一种快速，可重复且可靠的黑麦草种类鉴定方法，可用于一般种子批次的筛选。

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