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首页> 外文期刊>Biomaterials >Selective control of liver and kidney cells migration during organotypic cocultures inside fibronectin-coated rectangular silicone microchannels
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Selective control of liver and kidney cells migration during organotypic cocultures inside fibronectin-coated rectangular silicone microchannels

机译：在纤连蛋白包被的矩形有机硅微通道内进行器官型共培养期间肝和肾细胞迁移的选择性控制

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In this work, the behaviors of embryonic liver and kidney explants were studied inside rectangular polydimethylsiloxane (PDMS) microchannels. The organs were cultured under monoculture and coculture conditions on PDMS coated with or without fibronectin. The results demonstrated that the migration of cells from both organs is dependent on culture conditions and thus can be selectively controlled. In liver monocultures without fibronectin, cell migration in the microchannels resulted in the formation of a dense 3D tissue. Fibronectin reduced liver cell migration and enhanced the emergence of cells demonstrating typical hepatocyte phenotypes at the vicinity of the explant. The migration rate in liver liver cocultures, with and without fibronectin, was roughly twice the rate of cells under monoculture conditions. In cocultures, both livers merged to form a large tissue in which the two initial organs could not be identified. In kidney monocultures, with and without fibronectin, we did not observe any migration inside the microchannels. Contrary to liver cells, kidney cell migration was triggered when both fibronectin coating and coculture with liver or another kidney explant were used. The migration was more largely observed in coculture with liver when compared to kidney-kidney cocultures. In the case of liver-kidney coculture with fibronectin, the progression of the kidney cells inside the microchannels appears as a displacement of the entire kidney explant in the direction of the liver. The liver cells did not move in those cases. After contact, we observed a complete merging of both liver and kidney explants. In contrast, for liver-kidney cocultures without fibronectin, only the liver moved toward the kidney. (c) 2006 Elsevier Ltd. All rights reserved.
机译：在这项工作中，研究了矩形聚二甲基硅氧烷（PDMS）微通道内部胚胎肝和肾脏外植体的行为。在单培养和共培养条件下，在用或不用纤连蛋白包被的PDMS上培养器官。结果表明，来自两个器官的细胞迁移取决于培养条件，因此可以被选择性地控制。在没有纤连蛋白的肝脏单一培养物中，微通道中的细胞迁移导致形成密集的3D组织。纤连蛋白减少了肝细胞迁移，并增强了在外植体附近表现出典型肝细胞表型的细胞的出现。在有或没有纤连蛋白的条件下，肝肝共培养物中的迁移率大约是单培养条件下细胞迁移率的两倍。在共培养中，两个肝脏融合形成一个大组织，无法识别两个初始器官。在有或没有纤连蛋白的肾脏单一培养物中，我们没有观察到微通道内的任何迁移。与肝细胞相反，当同时使用纤连蛋白包被和与肝脏或其他肾脏外植体共培养时，会触发肾细胞迁移。与肾脏-肾脏共培养相比，在与肝脏共培养中观察到了更多的迁移。在肝-肾与纤连蛋白共培养的情况下，微通道内肾细胞的进展表现为整个肾脏外植体向肝脏方向的移位。在这些情况下，肝细胞不动。接触后，我们观察到肝脏和肾脏外植体完全融合。相反，对于没有纤连蛋白的肝肾共培养，只有肝脏向肾脏移动。 （c）2006 Elsevier Ltd.保留所有权利。

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