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首页> 外文期刊>Biomaterials >Evaluation of three-dimensional scaffolds prepared from poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits.
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Evaluation of three-dimensional scaffolds prepared from poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits.

机译：由聚（3-羟基丁酸酯-co-3-羟基己酸酯）制备的三维支架对同种异体软骨细胞生长的软骨修复的评估。

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摘要

Articular cartilage repair using tissue engineering approach generally requires the use of an appropriate scaffold architecture that can support the formation of cartilage tissue. In this investigation, the potential of three-dimensional scaffolds made of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) was evaluated in rabbit articular cartilage defect model. Engineered PHBHHx cartilage constructs inoculated in vitro with rabbit chondrocytes for 30 days were examined. Subsequently the constructs inoculated with chondrocytes for 10 days were selected for transplantation into rabbits. After 16 weeks of in vivo implantation, both the engineered cartilage constructs and the bare scaffolds were found to be filled the defects with white cartilaginous tissue, with the engineered constructs showing histologically good subchondral bone connection and better surrounding cartilage infusion. Owing to pre-seeded chondrocytes in the PHBHHx scaffolds, better surface integrality and more accumulation of extracellular matrix (ECM) including type II collagen and sGAG were achieved in the engineered cartilage constructs. The repaired tissues possessed an average compressive modulus of 1.58MPa. For comparison, the defects without repair treatments still showed defects with fibrous tissues. These results demonstrated that PHBHHx is a useful material for cartilage tissue engineering.
机译：使用组织工程学方法进行的关节软骨修复通常需要使用能够支持软骨组织形成的合适的支架结构。在这项研究中，在兔关节软骨缺损模型中评估了由聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）制成的三维支架的潜力。检查了体外用兔软骨细胞接种30天的工程化PHBHHx软骨构建体。随后，选择接种软骨细胞10天的构建体以移植到兔中。在体内植入16周后，发现工程软骨构造和裸露的支架都被白色软骨组织填补了缺损，工程构造显示出组织学上良好的软骨下骨连接和更好的周围软骨灌注。由于在PHBHHx支架中预先植入了软骨细胞，因此在工程化的软骨构建体中实现了更好的表面完整性和更多的细胞外基质（ECM）积聚，包括II型胶原蛋白和sGAG。修复后的组织的平均压缩模量为1.58MPa。为了进行比较，未经修复的缺损仍显示出纤维组织缺损。这些结果表明，PHBHHx是用于软骨组织工程的有用材料。

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