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首页> 外文期刊>Biomaterials >Sustained intraspinal delivery of neurotrophic factor encapsulated in biodegradable nanoparticles following contusive spinal cord injury.
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Sustained intraspinal delivery of neurotrophic factor encapsulated in biodegradable nanoparticles following contusive spinal cord injury.

机译：挫伤性脊髓损伤后，持续包囊内包封在可生物降解的纳米颗粒中的神经营养因子。

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摘要

Glial cell line derived neurotrophic factor (GDNF) induces neuronal survival and tissue repair after spinal cord injury (SCI). A continuous GDNF supply is believed to gain greater efficacy in the neural restoration of the injured spinal cord. Accordingly, nanovehicle formulation for their efficient delivery and sustained release in injured spinal cord was examined. We first used fluorescence-labeled bovine serum albumin (FBSA) loaded in biodegradable poly(lactic acid-co-glycolic acid) (PLGA) for intraspinal administration after SCI and for in vitro study. Our results showed that the preservation of PLGA-FBSA was observed in the injured spinal cord at 24h, and PLGA-FBSA nanoparticles were well absorbed by neurons and glia, indicating that PLGA as a considerable nanovehicle for the delivery of neuroprotective polypeptide into injured spinal cord. Furthermore, intraspinal injection of GDNF encapsulated in PLGA (PLGA-GDNF) nanoparticles into the injured spinal cord proximal to the lesion center had no effect on gliosis when compared to that observed in SCI rats receiving PLGA injection. However, local administration of PLGA-GDNF effectively preserved neuronal fibers and led to the hindlimb locomotor recovery in rats with SCI, providing a potential strategy for the use of PLGA-GDNF in the treatment of SCI.
机译：胶质细胞源性神经营养因子（GDNF）诱导脊髓损伤（SCI）后神经元存活和组织修复。连续的GDNF供应被认为在受伤脊髓的神经修复中获得更大的功效。因此，检查了纳米车辆制剂在受伤的脊髓中的有效递送和持续释放。我们首先将荧光标记的牛血清白蛋白（FBSA）装入可生物降解的聚乳酸-乙醇酸共聚物（PLGA）中，用于脊髓损伤后的椎管内给药和体外研究。我们的结果表明，在受伤的脊髓处24h观察到PLGA-FBSA的保存，并且PLGA-FBSA纳米颗粒被神经元和神经胶质良好吸收，表明PLGA作为将神经保护性多肽输送到受伤的脊髓中的重要纳米载体。 。此外，与接受PLGA注射的SCI大鼠相比，将PLGA中封装的GDNF（PLGA-GDNF）纳米粒子的脊髓内注射到损伤中心附近的受损脊髓中对胶质增生没有影响。然而，局部施用PLGA-GDNF可以有效地保存SCI大鼠的神经元纤维，并导致后肢运动恢复，这为使用PLGA-GDNF治疗SCI提供了潜在的策略。

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