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首页> 外文期刊>Sensor Letters: A Journal Dedicated to all Aspects of Sensors in Science, Engineering, and Medicine >Preparing of Hybrid Bio-Organic-Inorganic Composite Membrane for Amperometric Mediatorless Glucose Micro Biosensor
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Preparing of Hybrid Bio-Organic-Inorganic Composite Membrane for Amperometric Mediatorless Glucose Micro Biosensor

机译：电流型无介体葡萄糖微生物传感器杂化生物-有机-无机复合膜的制备

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摘要

An amperometric micro glucose biosensor based on the hybrid bio-organic-inorganic composite sensitive membrane by which electrostatic absorption of glucose oxidase (GOD) in polyaniline (PAN) film on iridium oxide (Ir_2O_3) film electrode has been fabricated. The microelectrode exhibited a linear relationship with the concentration of glucose in the range of 0.01 ～14 mM with a detection limit of 2.4 × 10~(-6) M. Iridium oxide electrode exhibits obviously electro-catalytic activity towards reduction of hydrogen peroxide (H_2O_2) at a low reductive potential. Ascorbic acid, uric acid and other oxidizable compounds coexisted (with glucose) in the sample did not cause interference to the determination. The selectivity, response time, reproducibility and lifetime of this micro biosensor are discussed. It has been used as a detector of flow-injection analysis (FIA) for glucose assay in blood serum directly.
机译：一种基于混合型生物-有机-无机复合敏感膜的安培型微葡萄糖生物传感器，通过该传感器制备了氧化铱（Ir_2O_3）膜电极上聚苯胺（PAN）膜中葡萄糖氧化酶（GOD）的静电吸收。微电极与葡萄糖浓度在0.01〜14 mM之间呈线性关系，检出限为2.4×10〜（-6）M。氧化铱电极对过氧化氢（H_2O_2）的还原具有明显的电催化活性。 ）在低还原电位下。样品中共存（与葡萄糖一起）的抗坏血酸，尿酸和其他可氧化的化合物不会干扰测定。讨论了该微型生物传感器的选择性，响应时间，重现性和寿命。它已被用作直接在血清中进行葡萄糖测定的流动注射分析仪（FIA）。

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