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首页> 外文期刊>Separation and Purification Technology >Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin
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Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin

机译：通过丝素蛋白的吸附分离橄榄叶提取物中的多酚

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摘要

General objective of the present work was to assess the isolation of polyphenols from olive leaves. The effects of extraction conditions on the total phenol content and antioxidant activity of olive leaf extract (OLE) were investigated. An extract with good antioxidant activity (7.52 mmol of Trolox equivalent antioxidant capacity (TEAC)/g olive leaf extract), and a high content of oleuropein (13.4%) and rutin (0.18%) could be obtained using 70% ethanol as extraction solvent. There was a good correlation between the antioxidant activity and the total phenol content. Furthermore, silk fibroin was used as a novel adsorbent to recover the polyphenols from the olive leaf extracts. The adsorbed amounts of rutin and oleuropein were 15 mg rutin/g silk fibroin and 96 mg oleuropein/g silk fibroin. Fraction consisting of mainly oleuropein and fraction rich in rutin, luteolin-7-glucoside, verbascoside, apigenin-7-glucoside were obtained by using silk fibroin filled column. Silk fibroin was found to be a promising adsorbent for the purification of oleuropein and rutin from olive leaf extracts.
机译：目前工作的总体目标是评估从橄榄叶中分离多酚的能力。研究了提取条件对橄榄叶提取物（OLE）的总酚含量和抗氧化活性的影响。以70％乙醇为萃取溶剂，可获得具有良好抗氧化活性的提取物（7.52 mmol Trolox当量抗氧化能力（TEAC）/ g橄榄叶提取物），高含量的橄榄苦苷（13.4％）和芦丁（0.18％） 。抗氧化剂活性与总酚含量之间具有良好的相关性。此外，丝素蛋白被用作新型吸附剂，以从橄榄叶提取物中回收多酚。芦丁和橄榄苦苷的吸附量为15mg芦丁/ g丝素蛋白和96mg橄榄苦苷/ g丝素蛋白。通过使用丝素蛋白填充柱获得主要由橄榄苦苷和富含芦丁的馏分，木犀草素-7-葡糖苷，马齿side甙，芹菜素-7-葡糖苷组成的馏分。发现丝素蛋白是从橄榄叶提取物中纯化橄榄苦苷和芦丁的有前途的吸附剂。

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