• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Separation and Purification Technology >Protein separation with magnetic adsorbents in micellar aqueous two-phase systems
【24h】

Protein separation with magnetic adsorbents in micellar aqueous two-phase systems

机译：胶束水相两相系统中磁性吸附剂分离蛋白质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We introduce a new concept for the purification and concentration of macromolecules such as proteins based on the use of magnetic adsorbents in concert with micellar aqueous two-phase systems. Aqueous solutions of certain nonionic surfactants can, depending on the prevailing temperature, separate into two co-existing phases, one enriched, the other depleted in surfactant. These are known as micellar aqueous two-phase systems. Hydrophilic proteins are normally excluded from the 'surfactant-rich' phase of micellar aqueous two-phase systems. We show here that by introducing magnetic microadsorbents into micellar aqueous two-phases the phase selectivity of a hydrophilic protein can be effectively inverted. The magnetic adsorbents adsorb the target protein and pull it out of the 'surfactant-depleted' phase into the 'surfactant-rich' phase from which it is normally excluded. Starting from a model solution containing a 1:1 ratio of the hen egg white proteins lysozyme and ovalbumin, the application of magnetic cation exchange micro-adsorbents combined with the nonionic surfactant Triton X-114 resulted in a target lysozyme yield of 74% and purity of >80%. The combination of selective magnetic adsorbents with micellar aqueous two-phase systems yields a highly tunable extraction system. Potentially, these so-called 'Magnetic Extraction Phases' provide the basis for flexible and easily scaled fractionation processes for hydrophilic proteins.
机译：我们介绍了一种新的概念，用于纯化和浓缩大分子，例如蛋白质，该方法基于磁吸附剂与胶束水两相系统的协同作用。某些非离子表面活性剂的水溶液可根据当时的温度分为两个共存相，一个是富集相，另一个是贫化了表面活性剂。这些被称为胶束水两相系统。亲水蛋白通常不包含在胶束水溶液两相系统的“富含表面活性剂”相中。我们在这里表明，通过将磁性微吸附剂引入胶束水两相中，可以有效地反转亲水蛋白的相选择性。磁性吸附剂吸附目标蛋白质，并将其从“表面活性剂耗尽”相中拉出到“富含表面活性剂”相中，通常不将其吸收。从含有1：1比例的鸡蛋清蛋白溶菌酶和卵清蛋白的模型溶液开始，将磁性阳离子交换微吸附剂与非离子表面活性剂Triton X-114结合使用可达到74％的目标溶菌酶产率和纯度> 80％。选择性磁性吸附剂与胶束水两相系统的组合产生了高度可调的萃取系统。这些所谓的“磁性萃取相”可能为亲水性蛋白质的灵活，易于规模化的分级分离工艺提供基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号