• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Separation and Purification Technology >Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography
【24h】

Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography

机译：超临界流体萃取-洗脱色谱法分离南极拟南芥中保护视域的玉米黄质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microalgae is a potent source of carotenoids and is regarded as functional foods consumed by people worldwide. In this study, supercritical fluid extraction coupled with elution chromatography was employed to recover six carotenoids including zeaxanthin from Nannochloropsis oculata and extraction efficiency of zeaxanthin was determined. The amount of the zeaxanthin (400 mg/g) in the elution fraction increased more than twenty folds from that of the SFE extract (13.17 mg/g). The extracts were subsequently subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay, 2-2'-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid (ABTS)) assay and adult retinal pigment epithelium-19 (ARPE-19) cells. Experimental results demonstrate that other components than zeaxanthin may play a crucial role in the anti-oxidative ability of the extracts from N. oculata. This work provides a useful method to recover bioactive anti-oxidative zeaxanthin from microalgae of N. oculata by using modified supercritical fluids extraction.
机译：微藻是类胡萝卜素的有效来源，被认为是全世界人们食用的功能性食品。在这项研究中，采用超临界流体萃取结合洗脱色谱法从七叶草中回收了包括玉米黄质在内的六种类胡萝卜素，并确定了玉米黄质的提取效率。洗脱部分中的玉米黄质（400 mg / g）比SFE提取物（13.17 mg / g）增加了二十倍以上。随后对提取物进行2,2-二苯基-2-吡啶并肼水合物（DPPH）测定，2-2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸（ABTS））测定中的抗氧化能力测试和成人视网膜色素上皮19（ARPE-19）细胞。实验结果表明，玉米黄质以外的其他成分可能在烟叶猪笼草提取物的抗氧化能力中起关键作用。这项工作提供了一种有用的方法，可以通过使用改良的超临界流体萃取从眼球藻的微藻中回收生物活性的抗氧化玉米黄质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号