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Ethanol precipitation and ultrafiltration of inulinases from Kluyveromyces marxianus

机译:乙醇沉淀和超滤马克斯克鲁维酵母中的菊粉酶

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摘要

Inulinase (EC 3.2.1.7) produced by Kluyveromyces marxianus NRRL Y-7571 by solid state fermentation of sugarcane bagasse was purified from culture broth by ethanol precipitation and ultrafiltration. The effects of the concentration of ethanol and the rate of addition of ethanol to the crude extract on the purification yield and purification factor were assessed using the experimental design technique. Precipitation caused an activation of enzyme and allowed purification factor up to 2-fold. After precipitation with ethanol 55% (v/v) and a flow rate of 10 mL/min, followed by ultrafiltration with a 100 kDa membrane, the inulinase could be purified by a factor of 5.5-fold with 86.1% yield.
机译:通过乙醇沉淀和超滤从培养液中纯化由马克斯克鲁维酵母NRRL Y-7571通过固态发酵生产的菊粉酶(EC 3.2.1.7)。使用实验设计技术评估了乙醇浓度和乙醇向粗提物中的添加速率对提纯产率和提纯因子的影响。沉淀引起酶的活化,并使纯化因子提高2倍。用55%(v / v)乙醇沉淀,流速为10 mL / min,然后用100 kDa膜超滤后,菊粉酶可以纯化5.5倍,产率为86.1%。

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