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Prediction of protein breakthrough behavior using simplified analytical solutions

机译：使用简化的分析解决方案预测蛋白质的突破行为

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The predictability of the breakthrough behavior of lysozyme on the strong cation exchanger SP Sepharose FF was evaluated using two different but related analytical solutions.The first solution,known as the Thomas equation,assumes that adsorption can be represented by a pseudo second-order reaction rate law which translates into a Langmuir isotherm at equilibrium.The second solution,known as the Bohart-Adams equation,assumes that adsorption can be described by a quasi-chemical rate law which reduces to a rectangular isotherm at equilibrium.The Bohart-Adams solution can be regarded as a hmiting form of the Thomas solution.The equilibrium and rate parameters embedded in the two solutions were extracted from batch experiments.Predictions using the two simplified solutions agreed with experimental breakthrough data.When the isotherm is highly favorable the simpler Bohart-Adams solution can be used as a practically useful modeling tool in lieu of the mathematically complicated Thomas solution.
机译：使用两种不同但相关的分析溶液评估了溶菌酶在强阳离子交换剂SP Sepharose FF上的突破行为的可预测性。第一种解决方案称为Thomas方程，假定吸附可以由拟二阶反应速率表示。第二个解称为Bohart-Adams方程，它假定吸附可以用准化学反应速率定律描述，该化学反应定律在平衡时还原为矩形等温线。Bohart-Adams解可以从批处理实验中提取了两种溶液中嵌入的平衡和速率参数，使用这两种简化溶液的预测与实验突破数据相吻合，等温线是高度有利的，则较简单的Bohart-Adams解决方案可以用作实用的建模工具，代替数学上复杂的托马斯·索鲁蒂奥。

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