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首页> 外文期刊>Separation and Purification Technology >A comparative study of chromatographic methods for separating chemical compounds from Spiranthes australis(R.Brown)Lindl roots
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A comparative study of chromatographic methods for separating chemical compounds from Spiranthes australis(R.Brown)Lindl roots

机译：色谱分离方法从南北香兰（L. Brown）根中分离化合物的比较研究

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摘要

A comparative study of application high-speed counter-current chromatography(HSCCC),and reversed-phase semi-preparative liquid chromatography(RPLC)for separating chemical compounds from Spiranthes australis(R.Brown)Lindl roots was investigated.Some parameters such as solvent system,flow rate of the mobile phase,separation time,solvent consuming,sample size,yields,recoveries and purities of the products were all investigated.The results indicated that three compounds were separated by HSCCC only in one run and less than 240 min with the purities and recoveries of over 97 and 91%,respectively,which were also isolated by RPLC with the purities of over 98.5% and recoveries of less than 75% using multiple chromatographic steps,and the total separation time and solvent consuming were about 7 and 13 times more than HSCCC.The obtained compounds were elucidated as 5-hydroxyl-7,4'-dimethoxy-flavone(1),5-hydroxyl-7,3',4'-dimethoxy-flavone(2),and 5-gamma,gamma-dimefhylallyl-8-[2-(2,6-dihydroxyphenyl)-3-dimethyl-but-2-enyol]-umbelliferon(3),a novel compound,based on spectroscopic analysis.
机译：研究了应用高速逆流色谱（HSCCC）和反相半制备液相色谱（RPLC）分离甜叶菊（Spranthes australis（R.Brown）Lindl根）中化合物的比较研究。某些参数如溶剂系统，流动相流速，分离时间，溶剂消耗量，样品量，收率，回收率和纯度均得到了研究。结果表明，HSCCC仅一次运行即可分离出三种化合物，在不到240分钟的时间内即可完成分离。纯度和回收率分别超过97％和91％，也通过RPLC分离，使用多个色谱步骤分离，纯度超过98.5％，回收率低于75％，总分离时间和溶剂消耗分别约为7和90％。比HSCCC高13倍，得到的化合物为5-羟基-7,4'-二甲氧基-黄酮（1），5-羟基-7,3'，4'-二甲氧基-黄酮（2）和5- γ，γ-二巯基烯丙基-8- [2-（2,6-二羟基苯）基于光谱分析的新型化合物yl）-3-二甲基-丁-2-烯醇]-伞形酮（3）。

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