The effect of protein-membrane interactions on filtration behavior in forced-flow electrophoresis of protein solutions

A.L.Ahmad; N.A.H.Hairul

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The effect of protein-membrane interactions on filtration behavior in forced-flow electrophoresis of protein solutions

机译：蛋白质溶液的强制流动电泳中蛋白质-膜相互作用对过滤行为的影响

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The experimental was carried out to study the filtration behavior in the filtration of proteins,bovine serum albumin(BSA)and bovine gamma-globulin(BGG)under the influence of electric field strength(E).The protein-membrane interactions play important roles in the filtration behavior during the process.The pH changed gradually during the operation time resulted in the changes in physicochemical properties of membrane and protein solutions.It was found that the lowest permeate flux occurred at the IEP of both BSA(pH 4.9)and BGG(pH 5.86-6.70).The high permeate flux was found to be at higher and lower than IEP of the proteins due to electrostatic interaction/repulsion between protein and membrane surface.

机译：实验研究了在电场强度（E）影响下蛋白质，牛血清白蛋白（BSA）和牛γ-球蛋白（BGG）的过滤行为。蛋白质-膜相互作用在蛋白质的过滤中起着重要的作用。 pH在操作期间逐渐变化，导致膜和蛋白质溶液的理化性质发生变化。发现BSA（pH 4.9）和BGG（IED）的IEP发生的渗透通量最低。 pH 5.86-6.70）。由于蛋白质和膜表面之间的静电相互作用/排斥作用，发现高渗透通量高于和低于蛋白质的IEP。

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《Separation and Purification Technology》 |2008年第3期|共7页
作者
A.L.Ahmad; N.A.H.Hairul;
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正文语种 eng
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Forced-flow electrophoresis; Protein-membrane interactions; Protein; Bovine serum albumin; Bovine gamma-globulin;

机译：强制流电泳;蛋白质-膜相互作用;蛋白质;牛血清白蛋白;牛γ-球蛋白;

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1. The effect of protein-membrane interactions on filtration behavior in forced-flow electrophoresis of protein solutions [J] . A.L.Ahmad, N.A.H.Hairul Separation and Purification Technology . 2008,第3期

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5. The role of protein-protein interactions in the phase behavior of aqueous protein solutions. [D] . Anderson, Camille O. 2001

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The effect of protein-membrane interactions on filtration behavior in forced-flow electrophoresis of protein solutions

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