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首页> 外文期刊>Separation and Purification Technology >Rapid large scale purification of ellagitannins from pomegranate husk,a by-product of the commercial juice industry
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Rapid large scale purification of ellagitannins from pomegranate husk,a by-product of the commercial juice industry

机译：从石榴皮中快速大规模纯化鞣花单宁，石榴皮是商业果汁行业的副产品

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摘要

Pomegranate(Punica granatum L.)fruits are widely consumed fresh and in processed forms as juice,jams and wine.Pomegranate fruit husk/peel is a rich source of hydrolyzable tannins called ellagitannins(ETs).In the commercial pomegranate juice(PJ)industry,these ETs are extracted from the husk in significant quantities into the juice due to their hydrophilic properties.Pomegranate husk,a by-product of the PJ industry,is therefore an inexpensive and abundant source of ETs.Previous methods to isolate pomegranate ETs included labor intensive and time-consuming solid phase extractions by column chromatography(C-18,polyamides,cellulose,Sephadex Lipophilic LH-20,Diaion HP20)and/or use of specialized instruments such as preparative-high performance liquid chromatography(HPLC).We have used an Amberlite XAD-16 resin vacuum-aspirated column to rapidly purify an aqueous extract of pomegranate husk to afford total pomegranate tannins(TPT)in substantial yields(58-60 g TPT/kg husk;time <1 h).Using analytical HPLC and tandem LC-ES/MS,evaluation of TPT showed that it contains the major fruit husk ET,punicalagin(80-85% w/w)and ellagic acid(EA;1.3% w/w)and unquantified amounts of punicalin and EA-glycosides(hexoside,rhamnoside and pentoside).Since pomegranate ETs are reported to show potent antioxidant,antiatherosclerotic and anticancer activities,this method can be used for the large-scale production of TPT for future in vitro and in vivo biological studies.This method is practical for industrial applications and could provide a low-cost means to use a currently underutilized food by-product to develop phytoceuticals with potential health benefits or to develop products for use in the cosmetic and food biopreservative industries.
机译：石榴（Punica granatum L.）水果被广泛食用，以果汁，果酱和葡萄酒的形式加工或加工。石榴果皮/果皮是可水解单宁的丰富来源，被称为鞣花单宁（ET）。在商业化石榴汁（PJ）行业中石榴皮是PJ行业的副产品，因此是廉价且丰富的ETs来源。分离石榴ETs的先前方法包括劳动通过柱色谱法（C-18，聚酰胺，纤维素，Sephadex亲脂性LH-20，Diaion HP20）和/或使用专用仪器（如制备型高效液相色谱法（HPLC））进行密集且费时的固相萃取。使用Amberlite XAD-16树脂真空吸气柱快速纯化石榴皮的水提物，以大量获得总石榴单宁（TPT）（58-60 g TPT / kg壳;时间<1小时）。经HPLC和串联LC-ES / MS分析，TPT分析表明其主要成分为果壳ET，punicalagin（80-85％w / w）和鞣花酸（EA; 1.3％w / w）以及未定量的punicalin由于据报道石榴ETs具有强效的抗氧化，抗动脉粥样硬化和抗癌活性，因此该方法可用于大规模生产TPT，以用于将来的体内和体外生物学研究。以及EA-糖苷（己糖苷，鼠李糖苷和戊糖苷）。该方法对于工业应用是实用的，并且可以提供低成本的手段来使用当前未充分利用的食品副产物来开发具有潜在健康益处的植物药，或者开发用于化妆品和食品生物防腐行业的产品。

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