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首页> 外文期刊>Separation and Purification Technology >Optimization of extraction conditions of antioxidant phenolic compounds from mashua(Tropaeolum tuberosum Ruiz & Pavon)tubers
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Optimization of extraction conditions of antioxidant phenolic compounds from mashua(Tropaeolum tuberosum Ruiz & Pavon)tubers

机译：马铃薯块茎抗氧化酚类化合物提取条件的优化

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摘要

Optimal conditions for the extraction of phenolic compounds,anthocyanins,flavanols and ORAC antioxidant activity for two mashua genotypes were determined using the conventional liquid-solid method.Phenolic contents and antioxidant activity were affected by the type of solvent,pH level,solvent-water ratio and extraction time.In general,no significant differences in total phenolic recovery and ORAC values were observed when 90% methanol or methanol/acetone/water(45/45/10),both with 0.1% HCl,were used.By contrast,the 90% methanol solution with 0.1% HCl extracted the highest level of anthocyanins,whereas the solvent mixture extracted the highest level of flavanols.The purified extracts from the mashua genotypes presented total phenolics and ORAC contents within the ranges 14.4-18.7 mg gallic acid equivalents/g mashua dry matter(DM)and 221-359 mu mol of trolox equivalents/g mashua DM,respectively.The HPLC-DAD phenolic profiles obtained for the purified phenolic extracts for both mashua genotypes showed differences in proportions(% total area at 280 and 520 nm)depending on the solvents selected in this study.The high content of phenolic compounds and antioxidant activity found in the purified extracts for both mashua genotypes indicated that mashua extracts might be considered as a potential source of nutraceuticals in the future.
机译：采用常规液固法确定了两种马斯亚基因型提取酚类化合物，花色素苷，黄酮醇和ORAC抗氧化活性的最佳条件。酚类含量和抗氧化活性受溶剂类型，pH值，溶剂水比的影响。一般而言，当使用0.1％HCl的90％甲醇或甲醇/丙酮/水（45/45/10）时，总酚回收率和ORAC值均无显着差异。 90％的甲醇溶液和0.1％的HCl提取的花青素含量最高，而溶剂混合物提取的黄酮醇含量最高。从mashua基因型纯化的提取物显示总酚和ORAC含量在14.4-18.7 mg没食子酸当量/ g mashua干物质（DM）和221-359 mol mol trolox当量/ g mashua DM。获得两种mashua基因组的纯化酚提取物的HPLC-DAD酚谱取决于本研究中所选择的溶剂，不同类型的比例显示出不同的比例（在280和520 nm处的总面积百分比）。两种mashua基因型的纯化提取物中酚类化合物的高含量和抗氧化活性表明，mashua提取物可能被认为将来可能成为营养保健品的潜在来源。

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