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首页> 外文期刊>Separation and Purification Technology >Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system
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Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system

机译：两相水相体系初步捕获蜡样芽孢杆菌衍生的环糊精糖基转移酶

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In this works, the polymer-salt aqueous two phase system (ATPS) which is polyethylene-glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (V_R), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), V_R of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%.
机译：在这项工作中，构建了聚合物盐水溶液两相系统（ATPS），即聚乙二醇（PEG）和柠檬酸钠，以从发酵液中纯化CGTase酶。研究了相组成，连接线长度（TLL），体积比（V_R），粗样品上样量，pH和添加氯化钠（NaCl）等参数对环糊精糖基转移酶（CGTase）分配行为的影响。研究表明，在19.0％PEG和11.5％柠檬酸盐系统上实现了CGTase酶纯化的最佳系统，TLL为38.89％（w / w），V_R为2.0，另外4％（w / w） NaCl和20％的pH 7.0粗负载。来自芽孢杆菌属的CGTase。通过ATPS将其部分纯化至16.3倍，产率为70％。

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