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首页> 外文期刊>Separation and Purification Technology >A study of human gamma-globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands
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A study of human gamma-globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands

机译：含不同氨基酸配体的PVDF中空纤维亲和膜对人γ球蛋白的吸附能力研究

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摘要

Affinity membranes were prepared with poly(vinylidene fluoride) (PVDF) hollow fibers or flat membranes as the matrix,1,6-hexanediamine (HDA) as the space arm,and amino acid-L-phenylalanine (L-Phe),L-tryptophane (L-Trp) and L-histidine (L-His) as the hydrophobia ligands,respectively.X-ray photoelectron spectroscopy (XPS) analysis results indicated that the contents of oxygen and nitrogen increased from 5.6% to 16.5% and 0.6% to 3.0% on the membrane surface after L-Phe modification,respectively,while fluoride content decreased from 38.3% to 23.0%.The effects of operation parameters such as pH value and ionic strength,on the human gamma-globulin (HGG) adsorption capacity of the affinity membranes were investigated in a batch system.The adsorption phenomena appeared to follow a typical Langmuir adsorption isotherm under the optimal condition,where the maximum adsorption capacity (q_m) of L-Phe affinity membrane for gamma-globulin was 0.318 mg/cm~2 membrane and the equilibrium constant (K_d) value was found to be 0.453 mg/mL solution from the Scatchard plot.Forty hollow fibers with a membrane area of 0.0188 m~2 in the membrane module adsorb 21 mg HGG from 10 mL human plasma with a purity of 83.9% in a single-pass mode.And the affinity membrane represented good stability throughout repeated adsorption-elution cycles.
机译：以聚偏二氟乙烯（PVDF）中空纤维或扁平膜为基质，1,6-己二胺（HDA）作为空间臂，氨基酸-L-苯丙氨酸（L-Phe），L-色氨酸（L-Trp）和L-组氨酸（L-His）分别为疏水配体。X射线光电子能谱（XPS）分析结果表明，氧和氮的含量从5.6％增加到16.5％和0.6％ L-Phe修饰后膜表面分别降低至3.0％，而氟化物含量从38.3％降低至23.0％.pH值和离子强度等操作参数对人γ-球蛋白（HGG）吸附容量的影响在最佳条件下，L-Phe亲和膜对γ-球蛋白的最大吸附量（q_m）为0.318 mg / cm，在最佳条件下，吸附现象遵循典型的Langmuir吸附等温线。 〜2膜和平衡常数（K_d在Scatchard图中发现溶液值为0.453 mg / mL.40个膜组件中膜面积为0.0188 m〜2的中空纤维从10 mL人血浆中吸附21 mg HGG，单次纯度为83.9％通过模式。亲和膜在重复的吸附-洗脱循环中表现出良好的稳定性。

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