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A global aproach of ultrafiltration of compelx biological solutions

机译：复杂生物溶液超滤的全球方法

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This paper deals with the filtration of a single protein (#beta#-lactoglobulin) solution in comparison with a mixture of yeast extract and #beta#-lactoglobulin. It intends to show how all the compounds of a compelx biological solution interfere with the results of filtration. two different membranes have been used: one constituted from organic regenerated cellulose and the other from zirconium oxide. Both membranes are studied with regard to selectivity (Transmission rate) and fluxes (Resistance model). It is shown that the filtration of a single protein like #beta#-lactoglobulin leads to the building of a dynamic layer at wall, the resistance and selectivity of which are conditioned by the interactions with the membrane support (specifically protein adsorption on inorgnaic material) and thecharacteristic behaviour of #beta#-lactoglobulin (loss of solubility at its isoelectric point). But this layer is altered when yeast extract is added. These modifications result in flux decrease and changes in selectivity. Adsorption of peptides and ionic strength variations due to charged species contained in yeast extract are supposed to be involved in these modifications. It is thus demonstrated that the choice of a membrane for the filtration of a real biological fluid can not be done easily be only considering the filtration of one signle compound.
机译：与酵母提取物和＃β＃-乳球蛋白的混合物相比，本文研究了单一蛋白质（＃beta＃-乳球蛋白）溶液的过滤。它旨在显示复合生物溶液中的所有化合物如何干扰过滤结果。已经使用了两种不同的膜：一种由有机再生纤维素构成，另一种由氧化锆构成。研究了两种膜的选择性（透过率）和通量（电阻模型）。结果表明，单个蛋白质（如＃beta＃-乳球蛋白）的过滤会导致在壁上形成动态层，其动态性和选择性受与膜载体的相互作用（特别是蛋白质吸附在无机材料上）的影响。 β-乳球蛋白的特性（等电点溶解度的损失）。但是，当添加酵母提取物时，这一层会改变。这些修改导致通量减少和选择性变化。这些修饰涉及肽的吸附和由于酵母提取物中所含带电物质引起的离子强度变化。因此证明，仅考虑一种信号化合物的过滤就不能容易地选择用于过滤实际生物流体的膜。

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